Publication: The Effect of Sterilization on the Characteristics of Silk Fibroin Nanoparticles
Authors
Asensio Ruiz, M. A. ; Fuster, M. G. ; Martínez Martínez, T. ; Montalbán, M. G. ; Cenis, J. L. ; Víllora Cano, Gloria ; Lozano-Pérez, A. A.
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Facultad de Química
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Publisher
MDPI
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DOI
https://doi.org/10.3390/polym14030498
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info:eu-repo/semantics/article
Description
© 2022 by the authors____ This document is the published version of a published work that appeared in final form in Polymers ____
This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0 ____
To access the final edited and published work see:
https://doi.org/10.3390/polym14030498
Abstract
In recent years, silk fibroin nanoparticles (SFNs) have been consolidated as drug delivery
systems (DDSs) with multiple applications in personalized medicine. The design of a simple, inexpensive,
and scalable preparation method is an objective pursued by many research groups. When
the objective is to produce nanoparticles suitable for biomedical uses, their sterility is essential. To
achieve sufficient control of all the crucial stages in the process and knowledge of their implications
for the final characteristics of the nanoparticles, the present work focused on the final stage of sterilization.
In this work, the sterilization of SFNs was studied by comparing the effect of different
available treatments on the characteristics of the nanoparticles. Two different sterilization methods,
gamma irradiation and autoclaving, were tested, and optimal conditions were identified to achieve
the sterilization of SFNs by gamma irradiation. The minimum irradiation dose to achieve sterilization
of the nanoparticle suspension without changes in the nanoparticle size, polydispersity, or Zpotential
was determined to be 5 kiloGrays (kGy). These simple and safe methods were successfully
implemented for the sterilization of SFNs in aqueous suspension and facilitate the application of
these nanoparticles in medicine.
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Citation
Polymers 2022, 14(3), 498
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