Publication: CircRNA PDE3B regulates tumorigenicity via the miR-136-5p/MAP3K2 axis of esophageal squamous cell carcinoma
Authors
Yue, Wei ; Ye, Yiwang ; Chen, Baokun ; Wu, Da ; Wang, He ; Hui, Gang
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-18-567
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Background. CircRNA has a covalently
closed circular conformation and a stable structure.
However, the exact role of circRNA in esophageal
squamous cell carcinoma (ESCC) remains uncertain.
The purpose of this study was to explore the role of
hsa_circ_0000277 (circ_PDE3B) in ESCC.
Methods. The expression levels of circ_PDE3B,
miR-136-5p and mitogen-activated protein kinase kinase
kinase 2 (MAP3K2) in ESCC tissues and cells were
detected by quantitative real-time polymerase chain
reaction (qRT-PCR) or western blot. The proliferation
ability of EC9706 and KYSE30 cells was detected by
clonal formation, 5-ethynyl-2’-deoxyuridine (EdU) and
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide (MTT) assays. Flow cytometry was
used to detect the apoptosis rate of cells. Transwell assay
was used to detect the invasion ability of EC9706 and
KYSE3 cells. The relationship between miR-136-5p and
circ_PDE3B or MAP3K2 was verified by dual-luciferase
reporter assay and RNA pull-down, and the effect of
circ_PDE3B on tumor growth in vivo was explored
through tumor transplantation experiment. Immunohistochemistry (IHC) assay was used to detect MAP3K2 and
Ki67 expression in mice tumor tissues.
Results. The results showed that circ_PDE3B was
highly expressed in ESCC tissues and cells. Downregulated circ_PDE3B expression in ESCC cells
significantly reduced cell proliferation, migration and
invasion. Circ_PDE3B served as a sponge for miR-136-
5p, and miR-136-5p inhibition reversed the roles of
circ_PDE3B knockdown in ESCC cells. MAP3K2 was a
direct target of miR-136-5p, and miR-136-5p targeted
MAP3K2 to inhibit the malignant behaviors of ESCC
cells. Furthermore, circ_PDE3B regulated MAP3K2
expression by sponging miR-136-5p. Importantly,
circ_PDE3B knockdown inhibited tumor growth in vivo.
Conclusions. In conclusion, circ_PDE3B acted as
oncogenic circRNA in ESCC and accelerated ESCC
progression by adsorption of miR-136-5p and activation
of MAP3K2, supporting circ_PDE3B as a potential
therapeutic target for ESCC.
publication.page.subject
Citation
Histology and Histopathology Vol. 38, nº9 (2023)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/