Publication: Age-related changes in mitochondrial membrane composition of rainbow trout (Oncorhynchus mykiss) heart and brain
Authors
Almaida Pagán, Pedro Francisco ; Costa, J. de ; Mendiola, P ; Tocher, D R
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.cbpb.2012.05.013
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info:eu-repo/semantics/article
Description
© 2012 Elsevier Inc. This document is the Published version of a Published Work that appeared in final form in Comparative Biochemistry and Physiology, Part B. To access the final edited and published work see https://doi.org/10.1016/j.cbpb.2012.05.013
Abstract
Membrane composition, particularly of mitochondria, could be a critical factor by determining the propaga-tion of reactions involved in mitochondrial function during periods of high oxidative stress such as rapid growth and aging. Considering that phospholipids not only contribute to the structural and physical properties of biological membranes, but also participate actively in cell signaling and apoptosis, changes affecting either class or fatty acid compositions could affect phospholipid properties and, thus, alter mitochondrial
function and cell viability. In the present study, heart and brain mitochondrial membrane phospholipid compositions were analyzed in rainbow trout during the four first years of life, a period characterized by rapid growth and a sustained high metabolic rate. Specifically, farmed fish of three ages (1-, 2- and 4-years) were studied, and phospholipid class compositions of heart and brain mitochondria, and fatty acid compositions of individual phospholipid classes were determined. Rainbow trout heart and brain mitochon-
dria showed different phospholipid compositions (class and fatty acid), likely related to tissue-specific functions. Furthermore, changes in phospholipid class and fatty acid compositions with age were also tissue-dependent. Heart mitochondria had lower proportions of cardiolipin (CL), phosphatidylserine (PS) and phosphatidylinositol, and higher levels of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) with age. Heart mitochondrial membranes became more unsaturated with age, with a significative increase of peroxidation index in CL, PS and sphingomyelin (SM). Therefore, heart mitochondria became more susceptible to oxidative damage with age. In contrast, brain mitochondrial PC and PS content decreased in 4-year-old animals while there was an increase in the proportion of SM. The three main phospholipid classes in brain (PC, PE and PS) showed decreased n−3 polyunsaturated fatty acids, docosahexaenoic acid and
peroxidation index, which indicate a different response of brain mitochondrial lipids to rapid growth and maturation.
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Citation
Comparative Biochemistry and Physiology, Part B, 2012, Vol. 163, pp. 129–137
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