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A novel in vivo model of focal light emitting diode-induced cone-photoreceptor phototoxicity: neuroprotection afforded by brimonidine, BDNF, PEDF or bFGF

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dc.contributor.authorOrtín Martínez, Arturo
dc.contributor.authorValiente Soriano, Francisco Javier
dc.contributor.authorGarcía Ayuso, Diego
dc.contributor.authorJiménez López, Manuel
dc.contributor.authorBernal Garro, José Manuel
dc.contributor.authorNieto López, Leticia
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorVillegas Pérez, Maria Paz
dc.contributor.authorWheeler, Larry A.
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAlarcón Martínez, Luis
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-01-27T08:31:04Z
dc.date.available2026-01-27T08:31:04Z
dc.date.copyright © 2014 Ortín-Martínez et al.
dc.date.issued2014-12-02
dc.description.abstractWe have investigated the effects of light-emitting diode (LED)-induced phototoxicity (LIP) on cone-photoreceptors and their protection with brimonidine (BMD), brain-derived neurotrophic factor (BDNF), pigment epithelium-derived factor (PEDF), ciliary neurotrophic factor (CNTF) or basic fibroblast growth factor (bFGF). In anesthetized, dark adapted, adult albino rats a blue (400 nm) LED was placed perpendicular to the cornea (10 sec, 200 lux) and the effects were investigated using Spectral Domain Optical Coherence Tomography (SD-OCT) and/or analysing the retina in oriented cross-sections or wholemounts immune-labelled for L- and S-opsin and counterstained with the nuclear stain DAPI. The effects of topical BMD (1%) or, intravitreally injected BDNF (5 µg), PEDF (2 µg), CNTF (0.4 µg) or bFGF (1 µg) after LIP were examined on wholemounts at 7 days. SD-OCT showed damage in a circular region of the superotemporal retina, whose diameter varied from 1,842.4±84.5 µm (at 24 hours) to 1,407.7±52.8 µm (at 7 days). This region had a progressive thickness diminution from 183.4±5 µm (at 12 h) to 114.6±6 µm (at 7 d). Oriented cross-sections showed within the light-damaged region of the retina massive loss of rods and cone-photoreceptors. Wholemounts documented a circular region containing lower numbers of L- and S-cones. Within a circular area (1 mm or 1.3 mm radius, respectively) in the left and in its corresponding region of the contralateral-fellow-retina, total L- or S-cones were 7,118±842 or 661±125 for the LED exposed retinas (n = 7) and 14,040±1,860 or 2,255±193 for the fellow retinas (n = 7), respectively. BMD, BDNF, PEDF and bFGF but not CNTF showed significant neuroprotective effects on L- or S-cones. We conclude that LIP results in rod and cone-photoreceptor loss, and is a reliable, quantifiable model to study cone-photoreceptor degeneration. Intravitreal BDNF, PEDF or bFGF, or topical BMD afford significant cone neuroprotection in this model.
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dc.identifier.citationPLoS One. 2014 Dec 2;9(12):e113798
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0113798
dc.identifier.eissn1932-6203
dc.identifier.urihttp://hdl.handle.net/10201/194149
dc.languageeng
dc.publisherPublic Library of Science
dc.relationThis work was supported by Fundación Séneca: 04446/GERM/07; Spanish Ministry of Economy and Competitiveness: SAF-2012-38328; ISCIII-FEDER: PI10/01496, PI10/00187; RETICS: RD07/0062/0001; and an unrestricted grant from Allergan Sales LLC. to MVS.
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113798
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.odsObjetivo 3: Salud
dc.titleA novel in vivo model of focal light emitting diode-induced cone-photoreceptor phototoxicity: neuroprotection afforded by brimonidine, BDNF, PEDF or bFGF
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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