Publication: Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes
| dc.contributor.author | Escrich, Ainoa | |
| dc.contributor.author | Moyano, Elisabeth | |
| dc.contributor.author | Cusido, Rosa M. | |
| dc.contributor.author | Bonfill, Mercedes | |
| dc.contributor.author | Hosseini, Bahman | |
| dc.contributor.author | Palazón, Javier | |
| dc.contributor.author | Almagro Romero, Lorena | |
| dc.contributor.department | Biología Vegetal | |
| dc.date.accessioned | 2025-01-29T15:51:24Z | |
| dc.date.available | 2025-01-29T15:51:24Z | |
| dc.date.issued | 2021-06 | |
| dc.description | © 2021 The Authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in Plant Physiology and Biochemistry. To access the final edited and published work see https://doi.org/10.1016/j.plaphy.2021.03.047 | |
| dc.description.abstract | Paclitaxel (PTX), a widely used anticancer agent, is found in the inner bark of several Taxus species, although at such low levels that its extraction is ecologically unsustainable. Biotechnological platforms based on Taxus sp. cell cultures offer an eco-friendlier approach to PTX production, with yields that can be improved by elicitation. However, the also limited excretion of target compounds from the producer cells to the medium hampers their extraction and purification. In this context, we studied the effect of treating T. media cell cultures with the elicitor coronatine (COR) and calix[8]arenes (CAL), nanoparticles that can host lipophilic compounds within their macrocyclic scaffold. The highest taxane production (103.5 mg.L−1), achieved after treatment with COR (1 μM) and CAL (10 mg.L−1), was 15-fold greater than in the control, and PTX represented 82% of the total taxanes analyzed. Expression levels of the flux-limiting PTX biosynthetic genes, BAPT and DBTNBT, increased after the addition of COR, confirming its elicitor action, but not CAL. The CAL treatment significantly enhanced taxane excretion, especially when production levels were increased by COR; 98% of the total taxanes were found in the culture medium after COR + CAL treatment. By forming complexes with PTX, the nanoparticles facilitated its excretion to the medium, and by protecting cells from PTX toxicity, its intra-and extra-cellular degradation may have been avoided. The addition of COR and CAL to T. media cell cultures is therefore a bio-sustainable and economically viable system to improve the yield of this important anticancer compound. | es |
| dc.format | application/pdf | es |
| dc.format.extent | 8 | es |
| dc.identifier.citation | Plant Physiology and Biochemistry, 2021, Vol. 163, pp. 68-75 | |
| dc.identifier.doi | https://doi.org/10.1016/j.plaphy.2021.03.047 | |
| dc.identifier.issn | Print: 0981-9428 | |
| dc.identifier.issn | Electronic: 1873-2690 | |
| dc.identifier.uri | http://hdl.handle.net/10201/149618 | |
| dc.language | eng | es |
| dc.publisher | Elsevier | es |
| dc.relation | This work was supported by the Spanish MEIC (BIO2017-82374-R) (AEI/FEDER, UE), the Generalitat de Catalunya (2017SGR242) and the Fundacion Seneca-Agencia de Ciencia y Tecnología de la Region de Murcia(19876/GERM/15). | es |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0981942821001716?via%3Dihub | |
| dc.rights | info:eu-repo/semantics/openAccess | es |
| dc.rights | Atribución 4.0 Internacional | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Calixarenes | es |
| dc.subject | Coronatine | es |
| dc.subject | Gene expression | es |
| dc.subject | Taxane production | es |
| dc.subject | Paclitaxel | es |
| dc.subject | Taxus cell cultures | es |
| dc.title | Improved biotechnological production of paclitaxel in Taxus media cell cultures by the combined action of coronatine and calix[8]arenes | es |
| dc.type | info:eu-repo/semantics/article | es |
| dspace.entity.type | Publication | es |
| relation.isAuthorOfPublication | 26b10f99-26d5-4ed0-a830-5b27d6665ae6 | |
| relation.isAuthorOfPublication.latestForDiscovery | 26b10f99-26d5-4ed0-a830-5b27d6665ae6 |
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