Publication: Lebein, a snake venom disintegrin, induces apoptosis in human melanoma cells
| dc.contributor.author | Hammouda, Manel B. | |
| dc.contributor.author | Zakraoui, Ons | |
| dc.contributor.author | Aloui, Zohra | |
| dc.contributor.author | Riahi-Chebbi, Ichrak | |
| dc.contributor.author | Karoui, Habib | |
| dc.contributor.author | Essafi Benkhadir, Khadija | |
| dc.contributor.author | Montenegro Arce, María Fernanda | |
| dc.contributor.author | Rodríguez López, José Neptuno | |
| dc.contributor.author | Sánchez del Campo Ferrer, Luis | |
| dc.contributor.department | Bioquímica y Biología Molecular A | es |
| dc.date.accessioned | 2024-12-10T09:10:34Z | |
| dc.date.available | 2024-12-10T09:10:34Z | |
| dc.date.issued | 2016-07-05 | |
| dc.description | © 2016 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Toxins. To access the final edited and published work see https://doi.org/10.3390/toxins8070206 | es |
| dc.description.abstract | Melanoma, the most threatening form of skin cancer, has a very poor prognosis and is characterized by its very invasive and chemoresistant properties. Despite the recent promising news from the field of immunotherapy, there is an urgent need for new therapeutic approaches that are free of resistance mechanisms and side effects. Anti-neoplasic properties have been highlighted for different disintegrins from snake venom including Lebein; however, the exact effect of Lebein on melanoma has not yet been defined. In this study, we showed that Lebein blocks melanoma cell proliferation and induces a more differentiated phenotype with inhibition of extracellular signal-regulated kinase (ERK) phosphorylation and microphthalmia-associated transcription factor (MITF) overexpression. Melanoma cells became detached but were less invasive with upregulation of E-cadherin after Lebein exposure. Lebein induced a caspase-independent apoptotic program with apoptosis inducing factor (AIF), BCL-2-associated X protein (BAX) and Bim overexpression together with downregulation of B-cell lymphoma-2 (BCL-2). It generated a distinct response in reactive oxygen species (ROS) generation and p53 levels depending on the p53 cell line status (wild type or mutant). Therefore, we propose Lebein as a new candidate for development of potential therapies for melanoma. | es |
| dc.format | application/pdf | es |
| dc.format.extent | 14 | es |
| dc.identifier.citation | Toxins, 2016, Vol. 8, N. 7: 206 | |
| dc.identifier.doi | https://doi.org/10.3390/toxins8070206 | |
| dc.identifier.issn | Electronic: 2072-6651 | |
| dc.identifier.uri | http://hdl.handle.net/10201/147241 | |
| dc.language | eng | es |
| dc.publisher | MDPI | es |
| dc.relation | This work was supported by grants from the Ministerio de Economia y Competitividad (MINECO; Co-financing with Fondos FEDER) (SAF2013-48375-C2-1-R) and the Fundación Séneca, the Región de Murcia (FS-RM) (19304/PI/14) to José Neptuno Rodríguez-López and by the Tunisian Ministry of Higher Education and Scientific Research (LR11IPT04) and the Institut Pasteur de Tunis to Khadija Essafi-Benkhadir. María F. Montenegro and Luis Sánchez-del-Campo were contracted by Fundación de la Asociación Española contra el Cáncer (FAECC). Manel B. Hammouda was supported by a grant of Erasmus mundus EU Mare Nostrum. | es |
| dc.relation.publisherversion | https://www.mdpi.com/2072-6651/8/7/206 | es |
| dc.rights | info:eu-repo/semantics/openAccess | es |
| dc.rights | Atribución 4.0 Internacional | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Lebein | es |
| dc.subject | Disintegrin | es |
| dc.subject | Snake venom | es |
| dc.subject | Melanoma | es |
| dc.subject | Apoptosis | es |
| dc.title | Lebein, a snake venom disintegrin, induces apoptosis in human melanoma cells | es |
| dc.type | info:eu-repo/semantics/article | es |
| dspace.entity.type | Publication | es |
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| relation.isAuthorOfPublication.latestForDiscovery | 2d4e44e6-9dda-4146-8aef-fe0c6b5a8b8f |
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