Publication: Alterations in the von Ebner's gland secretion and implications for taste sensation in diabetic (db/db) mice
Authors
Liao, Meng-Lin ; Kung, Hsiu-Ni ; Lu, Kuo-Shyan ; Shen, Jia-Hong ; Peng, Wei-Hao
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-379
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info:eu-repo/semantics/article
Description
Abstract
The taste buds and associated glands, known
as von Ebner's glands (VEGs), are involved in and
augment gustatory function. The obese diabetic db/db
mouse, which has defects in the leptin receptor, displays
enhanced neural responses to, and an elevated behavioral
preference for, sweet stimuli. However, the effect of
diabetes on the morphology of circumvallate papilla
(CVP) taste buds and the role of VEGs have not been
investigated. The present study aimed to compare the
CVP taste buds and VEGs in wildtype (Wt) and type 2
diabetic (db/db) mice. These mice were divided into
control and isoproterenol-treated (at 1 h, 2 h, and 4 h
after one day of fasting) groups, and were sacrificed for
morphometric, immunohistochemical, and ultrastructural
analyses. Morphometry revealed no significant
difference in papilla size and the number of taste buds in
the control and diabetic groups. Detection of PGP 9.5-
immunoreactivity revealed nerve fibers in the trench
wall of vallate papillae, but no significant differences
were detected between groups. α-Amylase immunoreactivity levels in Wt and db/db mice were also similar.
However, 1 h after isoproterenol injection, the majority
of the VEG secretion of db/db mice was discharged,
while the level of α-amylase was restored by 2 h after
injection. The effect on α-amylase was in line with the
quantitative ultrastructural analysis of the secretory
granules. Our findings suggest diabetic metabolic
disturbances in db/db mice do not alter the structure or
innervation of CVP taste buds. However, the VEG
secretory pattern was altered in db/db mice and might
disrupt taste sensation.
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Citation
Histology and Histopathology Vol. 37, nº1 (2022)
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