Publication: Morphological effects of oestradiol-17ß‚ and selective oestrogen receptor a and ß agonists on luteinising hormone-secreting
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Date
2008
Authors
Garrido-Gracia, José C. ; Gordon, Ana ; Aguilar, Rafaela ; Monterde, J. G. ; Blanco, A. ; Martín de las Mulas, J. ; Sánchez-Criado, J.E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Summary. To investigate the role played by the different
rat gonadotroph oestrogen receptor (ER) pools in the
effects of oestradiol-17ß (E2) on gonadectomy cells,
two-week ovariectomised (OVX) rats were used. The
basic experimental group of rats was injected with 3 mg
of the selective ER modulator tamoxifen (TX) on days
15-20 after OVX. Groups of TX-treated OVX rats were
additionally injected on days 18-20 after OVX with 10
µg oestradiol benzoate (EB), 1 mg of the selective ERα
agonist propylpyrazole triol (PPT), or 1 mg of the
selective ERß diarylpropionitrile (DPN). Negative and
positive control groups were OVX rats injected over six
days after OVX with 0.2 ml oil and EB, respectively. On
day 21 after OVX, anterior pituitary glands were
dissected out and divided into halves. One hemipituitary
was processed for light microscopy and
immunocytochemistry for ßLH subunit and progesterone
receptor (PR), and the other hemipituitary for
ultrastructural evaluation. Results showed that:
gonadotrophs were the only pituitary cell type
expressing PR; treatment with TX alone shrunk
gonadectomy cells and induced both reorganization of
membrane-enclosed intracellular organelles and PR
expression, and treatment with DPN or EB, but not PPT,
reduced the agonistic morphological effects of TX.
Considering that TX activates nuclear ERα, the results
indicate that activation of nuclear ERα is determinant
for the reversal effects of E2
on gonadotrope
morphology and PR expression, and the simultaneous
activation of ERß modulates the action of ERα in an
inhibitory fashion.
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