Publication: Analysis of gene status in cervical dysplastic lesions and squamous cell carcinoma using tissue microarrays
Loading...
Date
2009
Authors
Costa, C. ; Espinet, Blanca ; Molina, Miguel A. ; Salgado, Rocio ; Salido, M. ; Baró, Teresa ; Fusté, P. ; Mancebo, G. ; Carreras, R. ; Solé, Francesc ; Serrano, S. ; Alameda, F.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Cervical displasia are classified as CIN-I,
CIN-II and CIN-III. It has been observed that in at least
60% of CIN-I and CIN-II, the pathology disappears
spontaneously, while around 30% persist at 24 months,
10% progress to CIN-III and 1% develops as a SCC. The
factors involved in the evolution of the pathology are not
defined, although infection of HPV is a necessary
condition, but not the only one. For this reason, the
identification of genetic changes is an essential element
for understanding the carcinogenic process. It can also
serve as a helpful tool for identifying patients who may
be susceptible to its evolution and treatment, from
patients whose lesions could regress spontaneous and for
whom periodic follow-ups would be enough.
Fiftty three cervical biopsies from patients with
dysplasia and ISCC were included in the study. These
biopsies were set into nine macroarrays. Eight genes and
five proteins were examined in each samples (hTERT,
PIK3CA, hTERC, MYC, CCND1, BCL2, ZNF217 and
p16) by fluorescence in situ hybridization (FISH) and/or
immunohistochemistry (IHC).
The results reflected that the genetic alterations of
PIK3CA, ZNF217 and CCND1 were associated with the
evolution of normal tissue to CIN I, those of hTERC and
ERBB with the evolution of LSIL to HSIL, those of
hTERT and MYC with the evolution of CIN-II/CIN-III
to ISCC, and those of BCL-2 with the inception of
ISCC. With regards to proteins, the expression of MYC
and CCND1 in the initial stages of the illness would help
in the acquisition of the altered cellular phenotype.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.