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The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

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dc.contributor.authorGómez-Martín, Ángel
dc.contributor.authorBataller, Esther
dc.contributor.authorGomis Almendro, Jesús
dc.contributor.authorSánchez López, Antonio
dc.contributor.authorGadea Mateos, Joaquín
dc.contributor.authorVieira, Luis Alberto
dc.contributor.authorGarcía Roselló, Empar
dc.contributor.authorde la Fe Rodríguez, Christian
dc.contributor.authorGarcía-Galán Pérez, Ana
dc.contributor.departmentSanidad Animal
dc.date.accessioned2023-11-24T12:00:06Z
dc.date.available2023-11-24T12:00:06Z
dc.date.issued2020-05-13
dc.description© The Authors. 2020. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). This document is the Published version of a Published Work that appeared in final form in Animals. To access the final edited and published work see https://doi.org/10.3390/ani10050837es
dc.description.abstractMycoplasma bovis is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two M. bovis mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain M. bovis-free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of M. bovis through AI technologies. The viability of M. bovis (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a Lactobacillus spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 106 colony forming units (CFU)/mL or 3.24 × 108 CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of M. bovis, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (p < 0.05). Our results may support new strategies for reducing the risk of M. bovis transmission through AI.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.doihttps://doi.org/10.3390/ani10050837
dc.identifier.eisbnAnimals 2020, 10(5), 837es
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/10201/136006
dc.languageenges
dc.publisherMDPIes
dc.relationThis work was supported by the Spanish Ministry of Economy and Competitiveness (Spanish Government) and co-financed by FEDER funds, project AGL2016-76568-R.es
dc.relation.publisherversionhttps://www.mdpi.com/2076-2615/10/5/837es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectIn vitroes
dc.subjectViabilityes
dc.subjectMycoplasma bovises
dc.subjectDiluted bull semenes
dc.subjectEnrofloxacines
dc.subjectDoxycyclinees
dc.subjectLactobacillus spp.es
dc.titleThe Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semenes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication159edd48-a2f3-41a4-9b8c-622e4140a215
relation.isAuthorOfPublication.latestForDiscovery159edd48-a2f3-41a4-9b8c-622e4140a215
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