Publication: K-FGF protoncogene expression is associated with murine testicular teratogenesis, but is not involved during mouse testicular development
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Date
1997
Authors
Anta, J.M.de ; Monzó, M. ; Peris, B. ; Ruano, D.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The k-FGF gene, which belongs to the family
of the fibroblast growth factor genes, is implicated in
tumoral and developmental processes. It is expressed in
embryonal carcinoma cells, in embryonic stem cells,
during limb and tooth formation and in some germ cell
tumors. However, the expression of this protooncogene
during testicular development as well its relationship to
spontaneous teratogenesis have not been determined.
Here we investigate k-FGF expression during testicular
development in mice, as well as in a spontaneous
testicular teratoma (STT) and in the OTT6050
teratocarcinoma (TC) by Northern blotting, RT-PCR and
in situ hybridization. Severa1 data indicate that k-FGF
gene contains downstream regulatory sequences which
bind octamer factors. One of these transcription factors
which binds to k-FGF enhancer is Oct-4. Although the
k-FGF gene is activated by Oct-4 in embryonal
carcinoma and embryonic stem cells and Oct-4 is
expressed in the germ cells of the embryo, our results
indicate that there is no detectable k-FGF expression in
mouse testicular germ cells at any stage of development.
This indicates that Oct-4 does not activate transcription
of the k-FGF gene in mouse germ cells, and that k-FGF
is not implicated during testicular development. We also
show that there is a high k-FGF expression in the
experimental OTT6050 TC, but only very low levels in a
murine differentiated STT, suggesting that k-FGF activation may be responsible for the genesis and
development of STT, behaving as a marker of
malignancy in these neoplasms .
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