High-throughput DNA sequencing of microbiota at interproximal sites

Cardá-Diéguez, Miguel; Bravo González, Luis Alberto; Morata, Isabel María; Vicente, Ascensión; Mira, Alex

Publication:
High-throughput DNA sequencing of microbiota at interproximal sites

dc.contributor.author	Cardá-Diéguez, Miguel
dc.contributor.author	Bravo González, Luis Alberto
dc.contributor.author	Morata, Isabel María
dc.contributor.author	Vicente, Ascensión
dc.contributor.author	Mira, Alex
dc.contributor.department	Dermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned	2024-01-25T16:59:48Z
dc.date.available	2024-01-25T16:59:48Z
dc.date.issued	2020
dc.description	©2020. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published, version of a Published Work that appeared in final form in Journal of Oral Microbiology. To access the final edited and published work seehttps://doi.org/10.1080/20002297.2019.1687397	es
dc.description.abstract	Objective: The oral microbiota has been deeply studied by high-throughput sequencing techniques. However, although the interproximal regions have one of the highest caries rates in the oral cavity, information about the bacterial composition at those sites is scarce. Methods: In this study, we used 16S rRNA Illumina sequencing to describe the microbiota associated to interproximal regions at two time points. In addition, dental plaque samples at the vestibular and lingual surfaces from the same teeth were also analysed at the two time points. Results: Interproximal-associated microbiota was found to be similar to already described bacterial communities in other mouth niches. Streptoccocus, Veillonella, Rothia, Actinomyces, Neisseria, Haemophilus and Fusobacterium were the most abundant genera in this oral region. Statistical analyses showed that the microbiota from interproximal sites was more similar to that sampled from the vestibular surfaces than to the lingual surfaces. Interestingly, many potentially cariogenic bacteria such as Scardovia, Atopobium or Selenomonas were overrepresented in the interproximal regions in comparison with vestibular and lingual sites. Conclusion: The microbiota at interproximal regions appears to be specific and stable through time. Potentially pathogenic bacteria may increase caries development risk and gingival inflammation at those sites.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	Journal of Oral Microbiology 2020 12: 1687397
dc.identifier.doi	https://doi.org/10.1080/20002297.2019.1687397
dc.identifier.issn	2000-2297
dc.identifier.uri	http://hdl.handle.net/10201/137796
dc.language	eng	es
dc.relation	This work was supported by the Ministry of Science, Innovation and Universities [RTI2018-102032-B-100] and by the Department of Education, Research, Culture and Sport from Generalitat Valenciana [APOSTD/2018/081]	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.title	High-throughput DNA sequencing of microbiota at interproximal sites	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es