Publication: Effects of various antineoplastic agents on-2
Authors
García Reverte, Juana María ; Bernabeu-Esclapez, A. ; Muñoz Ramos, J. ; Vicente Ortega, Vicente ; Canteras Jordana, Manuel
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
An established human melanoma cell line
was treated with severa1 concentrations of three
antineoplastic drugs: melphalan (0.01 6, 0.032, 0.16 pn),
CCNU (1- (2-chloroethy1)-3-cyclohexyl1-- nitrosourea)
(0.04, 0.21, 0.42 pM) and 4-OHA (4-hydroxyanisole)
(4.01 x 1 0-4, 1 . 2 0~1 0 -2~.,4 ~ pM), and the effects on
cell growth and viability were compared.
24 hours after treatment, 4-OHA ( 1 ~ ~ ~ = 2 . 4 ~ 1 0 - ~
pM) was more cytotoxic than melphalan (ID50=0.016
pM) and CCNU (ID50=0.21 pM). However. after 96
hours exposure, the most effective drug was CCNU
(growth rate = -1.277), which caused the death of the
culture. This was followed by melphalan (growth rate =
- 1.024) and finally 4-OHA (growth rate= -0.69).
Similar ultrastructural cell injuries were observed
after the use of the three drugs: the dilation of
endoplasmic reticulum vesicles and the nuclear
membrane; mitochondria swelling; and the existence of
lamellar structures and cytoplasmic vacuoles.
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