Publication: Influence of examiners' experience on the reproducibility of different gold standard techniques and histological criteria for validation of the diagnosis of occlusal caries lesion
Authors
Simão Sarti, Caroline ; Schlesner de Oliveira, Renata ; Hernández de Campos, Priscila ; Fernandes Novaes, Tatiane ; Baffi Diniz, Michele ; Almeida Rodrigues, Jonas
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-047
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info:eu-repo/semantics/article
Description
Abstract
The aims of this study were to evaluate the
influence (1) of the examiner experience and (2) three
histological classification criteria on the reproducibility
of two gold standard techniques (non-dye or dye) for
validation of the diagnosis of occlusal caries. This study
comprised a sample of 210 digital images of 105
permanent teeth (105 rhodamine B dyed and 105 dyefree hemisections) and six examiners. Images were
evaluated on a laptop computer and categorised
according to three different histological classification
criteria (proposed by Ekstrand, Lussi or Downer) and
repeated in order to allow reproducibility calculation.
For data analysis, the six participants were divided into
two groups: G1: examiners with previous experience in
histological evaluation and G2: examiners with no
experience in histological evaluation. Statistical
significance was set at 0.05. Results. The mean intraexaminer reproducibility values in G1 were higher than
G2 in all variables. Intra-examiner reproducibility was
lower for the dye-free technique in both groups. The
mean values of inter-examiner reproducibility in G1
ranged from 0.60 to 0.68, and in G2 values ranged from
0.34 to 0.69. Conclusion. It can be concluded that: (1)
the examiners’ experience seems to influence the
reproducibility of the two gold standard techniques
studied and reproducibility tends to be lower when the
dye-free technique is used, and (2) the histological
classification criterion does not appear to influence the
reproducibility for validation of the diagnosis of occlusal
caries.
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Citation
Histology and Histopathology, Vol.34, nº4, (2019)
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