Publication: The influence of water pH on the genesis
of cadmium-induced cancer in a rat model
Authors
Nai, Gisele Alborghetti ; Golghetto, Gisele Maria Soria ; Estrella, Mariani Paulino Soriano ; Teixeira, Larissa Di Santi ; Moura, Felipe do Carmo ; Bremer Neto, Hermann ; Pariz, José Luiz Santos
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-30.61
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Cadmium is a heavy metal that is widely
used in industry and can cause tumours in multiple
organs. The purpose of our study was to investigate the
effect of water pH in the genesis of cadmium-induced
cancer. We divided 98 male Wistar rats into 7 groups:
group A - 15 rats that received cadmium chloride (CdCl
2 – 400 mg/L) in their drinking water at a neutral pH of
7.0; group B - 15 rats that received CdCl
2 (400 mg/L) in
their drinking water at an acidic pH of 5.0; group C - 15
rats that received CdCl
2 (400 mg/L) in their drinking
water at a basic pH of 8.0; group D - 15 rats that
received water at an acidic pH of 5.0; group E - 15 rats
that received water at a basic pH of 8.0; group F - 15 rats
that received water at a neutral pH of 7.0; and group G -
8 rats that were subcutaneously injected with a single
dose of cyclophosphamide (50 mg/kg). Groups A
through F were euthanised 6 months after the start of the
experiment and group G was euthanised 24 hours after
cyclophosphamide injection. We collected the liver,
kidneys, pancreas, prostate, seminal vesicles and testes
for histopathological analysis and the bone marrow for
micronuclei testing. In all of the groups, neither
neoplastic lesions nor an increase in micronuclei
(p>0.05) were observed in the liver, kidney, pancreas,
seminal vesicles and testes. We found that animals
exposed to cadmium had grade one prostatic
intraepithelial neoplasia, but this was found more
frequently in animals from group B (p<0.05). The acidic
pH increased the formation of pre-neoplastic lesions in
the prostate glands of cadmium-exposed animals.
publication.page.subject
Citation
Histology and Histopathology, Vol. 30, n.º 1 (2015)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/