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Development and validation of a time-resolved fluorescence immunoassay for the detection of anti-Toxoplasma gondii antibodies in goats

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dc.contributor.authorHuertas López, Ana
dc.contributor.authorMartínez Subiela, Silvia
dc.contributor.authorCerón, Jose J.
dc.contributor.authorVázquez Calvo, Ángela
dc.contributor.authorPazmiño Bonilla, Elvis Danilo
dc.contributor.authorLópez Ureña, Nadia María
dc.contributor.authorMartínez Carrasco, Carlos
dc.contributor.authorÁlvarez García, Gema
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-02-08T08:23:38Z
dc.date.available2024-02-08T08:23:38Z
dc.date.issued2021-04-21
dc.description©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Veterinary Parasitology. To access the final edited and published work seehttps://doi.org/10.1016/j.vetpar.2021.109432
dc.description.abstractToxoplasma gondii is a worldwide distributed parasite causing abortions and fetal malformations in small ruminants. The aim of this study was to design and validate a new immunoassay based on the use of TgSAG1-GRA8 chimeric antigen for the detection of anti-T. gondii antibodies in serum of goats. First, a time-resolved fluorescence immunoassay (TgSAG1-GRA8-TRFIA) was developed. In addition, the diagnostic performance of TgSAG1-GRA8-TRFIA was compared with an optimized enzyme-linked immunosorbent assay (TgSALUVET-ELISA) and a Western Blot (WB), both based on whole T. gondii tachyzoite antigenic extract. The TgSAG1-GRA8-TRFIA has shown a high intra- and inter-assay precision, analytical sensitivity and accuracy. The ROC analysis of this assay showed an optimal cut-off of 217.4 Units of Fluorometry for T. gondii (UFT), with 92 % of sensitivity and 90.48 % of specificity. A positive and statistically significant Spearman’s correlation with TgSALUVET-ELISA was detected, and kappa value was 0.83, presenting high agreement with both methods. However, TgSAG1-GRA8 protein showed cross-reactivity with specific anti-Neospora caninum antibodies. Thus, TgSAG-1-GRA8 chimeric antigen seems not to be an ideal option for the serodiagnosis of T. gondii infection in goats unless combined with the serodiagnosis of N. caninum infection in parallel. In the light of the results obtained, a comprehensive study on the existence of cross-reactivities between T. gondii antigens used in serological tests employed in animal health and specific antibodies directed against Toxoplasmatinae parasites should be performed.es
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dc.identifier.citationVeterinary Parasitology 293 (2021): 109432
dc.identifier.doihttps://doi.org/10.1016/j.vetpar.2021.109432
dc.identifier.issnPrint: 0304-4017
dc.identifier.urihttp://hdl.handle.net/10201/138948
dc.languageenges
dc.publisherElsevier
dc.relationSeneca Foundation of Murcia Region, Spain (project 19894/GERM/15)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectTime-resolved fluorescence immunoassayes
dc.subjectGoates
dc.subjectSerodiagnosises
dc.subjectToxoplasma gondiies
dc.subjectTgSAG1-GRA8 proteines
dc.titleDevelopment and validation of a time-resolved fluorescence immunoassay for the detection of anti-Toxoplasma gondii antibodies in goatses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublicationes
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