Publication: The ultrastructural effects of long-term
use of henna on the albino rat skin
Authors
Al-Shobaili, Hani A. ; El-Bassouny, Dalia R.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Tattooing with henna is a routine practice in
the Arab world. To the best of our knowledge, no
previous studies have evaluated the adverse histological
effects following henna tattooing on the ultrastructure of
the skin. The objectives of this study were to diagnose
the cytopathological alterations induced by commercial
henna and to investigate the adverse role of henna when
combined with sun ray on the skin. The skin of albino
rats was tattooed with natural and black henna for three
months, skin samples were examined by transmission
electron microscope. In addition, the concentration of
lead in henna samples was estimated by using atomic
absorption spectrophotometry. The results expanded the
understanding of the pathogenesis of henna-induced
phytophotodermatitis. We hypothesized that hennaassociated
additives penetrated the epidermal barrier to
gain access to the vascular dermis where the harmful
ingredients became concentrated, leading to skin
pathology through a dual mechanism. First, these
ingredients became re-transported into the epidermis
through vesicular trafficking leading to dermo-epidermal
blistering and cytoplasmic vacuolization of the stratum
basal cells. Following this, cytoplasmic vacuoles poured
their content into the nuclei through continuities with the
perinuclear cisterna, possibly leading to genetic
mutation. The progression of keratinocytes into the next
layers became associated with nuclear and cytoplasmic
signs of apoptosis with subsequent phagocytosis in other
epidermal cells, most probably keratinocytes. The
second mechanism of injury was mediated through
accumulation of inflammatory cells around capillaries in
the dermis with the release of angiogenic and mitogenic
mediators resulting in vasculopathy.
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Citation
Histology and Histopathology, vol. 29, nº 3 (2014)
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