Publication: Double immunofluorescence labeling for
CD31 and CD105 as a marker for polyether
polyurethane-induced angiogenesis in mice
Authors
Figueiredo, Camila Couto ; Braga Pereira, Núbia ; Pereira, Luciana Xavier ; Machado Oliveira, Laser Antônio ; Peixoto Campos, Paula ; Passos Andrade, Silvia ; Moro, Luciana
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-038
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info:eu-repo/semantics/article
Description
Abstract
A crucial component of the integration
between foreign implants and the host is angiogenesis.
However, to date, none of the available techniques and/or
endothelial markers employed to assess angiogenesis in
the implant/host interface seems to be able to highlight
vascular structures convincingly. In the present study we
investigated and compared the expression of two
endothelial cell markers: platelet endothelial cell
adhesion molecule (PECAM-1) (CD31) and endoglin
(CD105) using immunohistochemistry (IHC) and
immunofluorescence (IF) to identify and quantify newly
formed blood vessels in subcutaneous implants of
polyether-polyurethane sponge of formalin-fixed
paraffin-embedded tissue. At day 14 post implantation
the discs of the synthetic matrix were removed and
processed for histological and morphometric analysis. In
IHC staining for CD31 antibody the number of vessels
was 2.27±0.69 and 5.25±0.46 for CD105. In IF for CD31
the number of vessels was 15.36±1.295 and
10.54±0.8213 for CD105. The level of cross-reaction was
lesser in IF images compared with IHC images. Colocalization of CD31/CD105 using confocal images
showed positive correlation (Pearson's co-relation and
Manders’ equation). The double labeling for blood
vessels using the IF technique for CD31/CD105 may be
an important tool for evaluation of angiogenesis in
biomaterial/host integration.
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Citation
Histology and Histopathology, Vol.34, nº3, (2019)
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