On the metal cofactor in the Tyrosinase family

Solano, Francisco

Publication:
On the metal cofactor in the Tyrosinase family

dc.contributor.author	Solano, Francisco
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2021-03-25T20:46:39Z
dc.date.available	2021-03-25T20:46:39Z
dc.date.issued	2018-02-23
dc.description.abstract	The production of pigment in mammalian melanocytes requires the contribution of at least three melanogenic enzymes, tyrosinase and two other accessory enzymes called the tyrosinase-related proteins (Trp1 and Trp2), which regulate the type and amount of melanin. The last two proteins are paralogues to tyrosinase, and they appeared late in evolution by triplication of the tyrosinase gene.Tyrosinase is a copper-enzyme, and Trp2 is a zinc-enzyme. Trp1 has been more elusive, and the direct identification of its metal cofactor has never been achieved. However, due to its enzymatic activity and similarities with tyrosinase, it has been assumed as a copper-enzyme. Recently, recombinant human tyrosinase and Trp1 have been expressed in enough amounts to achieve for the first time their crystallization. Unexpectedly, it has been found that Trp1 contains a couple of Zn(II) at the active site This review discusses data about the metal cofactor of tyrosinase and Trps. It points out differences in the studied models, and it proposes some possible points accounting for the apparent discrepancies currently appearing. Moreover, some proposals about the possible flexibility of the tyrosinase family to uptake copper or zinc are discussed.	es
dc.format	application/pdf	es
dc.format.extent	17	es
dc.identifier.citation	International Journal of Molecular Sciences, 2018, 19, 633
dc.identifier.doi	10.3390/ijms19020633
dc.identifier.issn	1661-6596
dc.identifier.issn	1422-0067
dc.identifier.uri	http://hdl.handle.net/10201/105661
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	Project 19785/GERM/15 from Seneca Foundation, Murcia (Spain)	es
dc.relation.publisherversion	www.mdpi.com/journal/ijms	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.subject	Tyrosinase-related proteins	es
dc.subject	tyrosinase	es
dc.subject	melanin biosynthesis	es
dc.subject	copper	es
dc.subject	zinc	es
dc.subject	metal enzymes	es
dc.subject	adquisition protein structure	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología	es
dc.title	On the metal cofactor in the Tyrosinase family	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es