Antitumor activity of rosmarinic acid-loaded silk fibroin nanoparticles on HeLa and MCF-7 cells

Fuster, Marta G.; Carissimi, Gúzmán; García Montalbán, Mercedes; Víllora Cano, Gloria

Publication:
Antitumor activity of rosmarinic acid-loaded silk fibroin nanoparticles on HeLa and MCF-7 cells

dc.contributor.author	Fuster, Marta G.
dc.contributor.author	Carissimi, Gúzmán
dc.contributor.author	García Montalbán, Mercedes
dc.contributor.author	Víllora Cano, Gloria
dc.contributor.department	Ingeniería Química	es
dc.date.accessioned	2025-09-03T08:54:58Z
dc.date.available	2025-09-03T08:54:58Z
dc.date.issued	2021-09-18
dc.description	© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/.This document is the Published version of a Published Work that appeared in final form in Polymers. To access the final edited and published work see https://doi.org/ https://doi.org/10.3390/polym13183169	es
dc.description.abstract	Rosmarinic acid (RA), one of the most important polyphenol-based antioxidants, has drawn increasing attention because of its remarkable bioactive properties, including anti-inflammatory, anticancer and antibacterial activities. The aim of this study was to synthesize and characterize RA-loaded silk fibroin nanoparticles (RA-SFNs) in terms of their physical–chemical features and composition, and to investigate their antitumor activity against human cervical carcinoma and breast cancer cell lines (HeLa and MCF-7). Compared with the free form, RA bioavailability was enhanced when the drug was adsorbed onto the surface of the silk fibroin nanoparticles (SFNs). The resulting particle diameter was 255 nm, with a polydispersity index of 0.187, and the Z-potential was −17 mV. The drug loading content of the RA-SFNs was 9.4 wt.%. Evaluation of the in vitro drug release of RA from RA-SFNs pointed to a rapid release in physiological conditions (50% of the total drug content was released in 0.5 h). Unloaded SFNs exhibited good biocompatibility, with no significant cytotoxicity observed during the first 48 h against HeLa and MCF-7 cancer cells. In contrast, cell death increased in a concentration-dependent manner after treatment with RA-SFNs, reaching an IC50 value of 1.568 and 1.377 mg/mL on HeLa and MCF-7, respectively. For both cell lines, the IC50 of free RA was higher. The cellular uptake of the nanoparticles studied was increased when RA was loaded on them. The cell cycle and apoptosis studies revealed that RA-SFNs inhibit cell proliferation and induce apoptosis on HeLa and MCF-7 cell lines. It is concluded, therefore, that the RA delivery platform based on SFNs improves the antitumor potential of RA in the case of the above cancers.	es
dc.format	application/pdf	es
dc.format.extent	20	es
dc.identifier.citation	Polymers 2021, 13, 3169
dc.identifier.doi	https://doi.org/10.3390/polym13183169
dc.identifier.issn	Electronic: 2073-4360
dc.identifier.uri	http://hdl.handle.net/10201/158078
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	This work has been partially supported by the European Commission (FEDER/ERDF) and the Spanish MINECO (Refs. CTQ2017-87708-R and PID2020-113081RB-I00) through the Spanish State Research Agency, and the research support program of the Seneca Foundation of Science and Technology of Murcia, Spain (Ref. 20977/PI/18). Marta G. Fuster acknowledges support from Spanish MINECO (FPI grant, PRE2018-086441). Mercedes G. Montalbán acknowledges support from the University of Murcia and Santander Bank through the research project (RG2020-002UM) associated with her postdoctoral contract (Contract of generational renovation for research support of the University of Murcia).	es
dc.relation.publisherversion	https://www.mdpi.com/2073-4360/13/18/3169	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Rosmarinic acid	es
dc.subject	Silk fibroin	es
dc.subject	Nanoparticle	es
dc.subject	Antitumor activity	es
dc.subject	Cell viability	es
dc.subject	Cellular uptake	es
dc.subject	Cell cycle	es
dc.subject	Apoptosis	es
dc.title	Antitumor activity of rosmarinic acid-loaded silk fibroin nanoparticles on HeLa and MCF-7 cells	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es