Publication: Peg-and-socket junctions between smooth muscle cells
and endothelial cells in femoral veins are stimulated to
angiogenesis by prostaglandin E2 and glycerols
Authors
Díaz-Flores Jr., L. ; Gutiérrez, R. ; Sáez, F.J. ; Valladares, F. ; Villar, J. ; Díaz-Flores, L. ; Madrid Cuevas, Juan Francisco
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The administration of prostaglandin (PG) E2,
triacetylglycerol and glycerol induce the formation of
numerous vascular buds arising from the femoral vein,
as previously demonstrated by our group. In the present
study, a great number of peg-and-socket junctions (PSJs)
between smooth muscle cells (SMCs) (providing the
pegs) and ECs (forming the sockets) were demonstrated.
At the first stage, days 1 to 3, PSJs connect
subendothelial penetrating processes from activated
SMCs with activated ECs of the intima. Subsequently,
during angiogenesis (days 4 to 6), SMCs, showing
transitional aspects with pericytes, also form PSJs with
intimal ECs, but also new PSJs between SMCs and
sprouting ECs in the media layer were now observed.
Immunohistochemically, α-smooth muscle actin (α-
SMA) and H-caldesmon are positive in the cytoplasm of
the SMCs, showing a higher expression in pegs. Desmin,
however, although it is also positive in the cytoplasm of
the SMCs, is negative in the pegs. The expression of
CD34 in ECs reveals abundant positive folding that
appears to correspond to the sockets. The peculiar
expression of caldesmon, whose isoforms may
contribute to the regulation of cell motility, and to
vasculogenesis and angiogenesis, may have a role in the
different mechanisms by which PSJs act in the vein wall.
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