Publication: Expression of E-cadherin-catenin
complex in human benign schwannomas
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Date
2002
Authors
Hasegawa, M. ; Muramatsu, N. ; Tohma, Y. ; Fukaya, K. ; Fujisawa, H. ; Hayashi, Yoshihiro ; Tachibana, Osamu ; Kida, S. ; Yamashita, J. ; Saito, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The Ca2+-dependent cell adhesion molecule
E-cadherin has been known to express in normal and
reactive Schwann cells in rodents, and to play an
important role in Schwann cell-Schwann cell adhesion
and maintenance of peripheral nervous tissue
architecture. However, little is known about expression
of E-cadherin in schwannomas. The aim of the present
study was to investigate the cellular expression and
localization of E-cadherin, and its associated protein,
alpha E-, alpha N- and beta-catenins in human
schwannomas, which are supposed to derive from
Schwann cells. We tested the hypothesis that these
proteins might show an altered expression/distribution in
schwannoma cells which correlates with their neoplastic
behavior, including sparse cell-cell contact, as seen those
in meningiomas and various carcinomas. In human
schwannomas, however, E-cadherin, alpha E-catenin,
and beta-catenin were detected by western blotting and
i m m u n o h i s t o c h e m i s t r y, whereas alpha N-catenin was
not. Immunoprecipitation using anti-E-cadherin antibody
resulted in alpha E-catenin forming a complex with Ecadherin.
SSCP analysis revealed no mutations in the
transmembrane domain or in intracellular cateninbinding
site of E-cadherin. These data suggest that the Ecadherin-
alpha E-catenin complex is well preserved in
human schwannoma cells, which is compatible with its
benign behavior, and these molecules might be used as
additional cell markers of Schwann cell-derived tumors.
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