Publication: Co-expression of trypsin and tumour-associated
trypsin inhibitor (TATI) in colorectal adenocarcinomas
Authors
Solakidi, S. ; Tiniakos, D.G. ; Petraki, K. ; Stathopoulos, G.P. ; Markaki, I. ; Androulakis, G. ; Sekeris, C.E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Trypsin and its specific inhibitor, TATI
(tumour-associated trypsin inhibitor), are expressed in
normal human pancreas and in a variety of tumours. The
aim of the present study was to assess the parallel
expression of trypsin and TATI in colorectal cancer, in
comparison with their expression in normal epithelial
tissue, since proteases and their inhibitors are thought to
be co-expressed in malignant neoplasms. We also
assessed the possible significance of their expression as
a means of differentiation between normal and malignant
tissue. We examined qualitatively and semiquantitatively
the immunohistochemical expression of
trypsin and TATI on paraffin-embedded serial tissue
sections from 91 colorectal adenocarcinomas. The
reverse-transcriptase-polymerase-chain reaction (RTPCR)
was also performed on fresh malignant tissue from
55 of the above adenocarcinomas. Normal and nonmalignant
tissues adjacent to the tumours were also
evaluated. Cytoplasmic expression of trypsin (more than
25% of the cancer cells positive) was found in 67
(73.6%) adenocarcinomas, whereas TATI was expressed
in the cytoplasm of 59 (64.8%) cases studied. Statistical
analysis using Spearman’s test has demonstrated a
significant correlation between trypsin and TATI
immunohistochemical expression (p<0.01). RT-PCR
showed co-expression of trypsin and TATI mRNA in all
carcinomas studied. Distinct patterns of trypsin and
TATI immunohistochemical expression were observed in
adjacent, non-malignant tissues, where both trypsin and
TATI mRNA were also detected. Normal tissues were
negative by immunohistochemistry. Our results indicate
co-expression of trypsin and TATI in colorectal tumours
both at the mRNA and protein level. We conclude that in
colorectal neoplasms, high levels of trypsin and TATI
may be important for malignant tumour formation and/or
metastatic process.
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