Publication: Recent development of in vivo cryotechnique to cryobiopsy for living animals
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Date
2007
Authors
Ohno, N. ; Terada, N. ; Saitoh, S. ; Zhou, H. ; Fuji, Y. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Various microscopic methods have been used
to analyze the morphology and molecular distribution of
cells and tissues. Using conventional procedures,
however, ischemic or anoxic artifacts are inevitably
caused by tissue-resection or perfusion-fixation. The in
vivo cryotechnique (IVCT) was developed to overcome
these problems, and was found to be useful with light
microscopy for analyses of the distribution of watersoluble
molecules without anoxic effects at high time
resolution. But there are limitations to the application of
IVCT, such as exposure of target organs of living small
animals and immunoreactivity of lipid-soluble molecules
owing to freeze-substitution with acetone. Recently, a
new cryotechnique called “cryobiopsy” has been
developed, which enables one to obtain tissue specimens
of large animals including humans without ischemia or
anoxia, and has almost the same technical advantages as
IVCT. Both IVCT and cryobiopsy complement other
live-imaging techniques, and are useful for not only the
morphological observation of cells and tissues under
normal conditions, but also the preservation of all
components in frozen tissue specimens. Therefore,
morphofunctional information in vivo would be obtained
by freeze-substituion for light or electron microscopy,
and also by other analytical methods, such as freezefracture
replication, X-ray microanalyses, or Raman
microscopy. Considering the merits of both IVCT and cryobiopsy, their application should be expanded into
other microscopic fields and also from experimental
animal studies to clinical medicine.
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