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Melanopsin-containing or non-melanopsin–containing retinal ganglion cells response to acute ocular hypertension with or without brain-derived neurotrophic factor neuroprotection

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dc.contributor.authorRovere, Giuseppe
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorWang, Jiawei
dc.contributor.authorBernal-Garro, José M.
dc.contributor.authorGarcía-Carrillo, Nuria
dc.contributor.authorVillegas Pérez, Maria Paz
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultad de Medicina
dc.date.accessioned2026-01-27T19:42:23Z
dc.date.available2026-01-27T19:42:23Z
dc.date.copyright© 2016, Association for Research in Vision and Ophthalmology
dc.date.issued2016-12
dc.description.abstractPURPOSE. To analyze the responses of different retinal ganglion cell (RGC) types to acute ocular hypertension (AOH) and intravitreal administration of brain-derived neurotrophic factor (BDNF). METHODS. In adult albino rats, the anterior chamber of the left eye was cannulated with a needle connected to a saline container elevated 1½ meters above the eye for 75 minutes. Rats received 12 hours before a 5 ul intravitreal injection containing 5 ug BDNF in 1% albumin PBS or vehicle and were analyzed 3, 7, 14, or 45 days later. Both retinas were dissected as wholemounts and immunolabeled for melanopsin (to identify intrinsically photosensitive RGCs) or Brn3a (to identify all RGCs except melanopsin +RGCs). RESULTS. During AOH there is ischemic damage and mechanical eye-globe deformation. Acute ocular hypertension results in a progressive loss of Brn3a+RGCs in the vehicle-treated retinas (39%, 35%, 25%, and 13% of the original value, at 3, 7, 14, or 45 days, respectively), whereas BDNF increases their survival to 81%, 73%, 59%, or 57% at the same time periods. In vehicletreated retinas, 37% or 39% of m+RGCs survive at 14 or 45 days, respectively, whereas BDNF treatment increases their survival to 40% or 78% at the same time points. CONCLUSIONS. Different types of RGCs respond differently to AOH because Brn3a+RGCs die progressively, but m+RGCs do not. After a transient downregulation of melanopsin expression, their number remains constant and their survival is proportionally higher than that of Brn3a+RGCs. BDNF affords a permanent protection up to 45 days after AOH injury inboth types of RGCs.
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dc.identifier.citationInvest Ophthalmol Vis Sci. 2016 Dec 1;57(15):6652-6661.
dc.identifier.doihttps://doi.org/10.1167/iovs.16-20146
dc.identifier.urihttp://hdl.handle.net/10201/195509
dc.languageeng
dc.publisherAssociation for Research in Vision and Ophthalmology
dc.relationSupported by Fundación Séneca, Agencia de Ciencia y Tecnología Region de Murcia (19881/GERM/15), and the Spanish Ministry of Economy and Competitiveness, Instituto de Salud Carlos III, Fondo Europeo de Desarrollo Regional ‘‘Una Manera de Hacer Europa’’ (SAF2015-67643-P, PI13/01266, RD12/0034/0014, PI13/00643).
dc.relation.publisherversionhttps://iovs.arvojournals.org/article.aspx?articleid=2592946
dc.rightsAttribution-NonCommercial-NoDerivates 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectRat retinal ganglion cells
dc.subjectAcute ocular hypertension
dc.subjectIntrinsically photosensitive RGCs
dc.subjectBDNF neuroprotection
dc.subjectMelanopsin retinal ganglion cells
dc.subjectAdult albino rats
dc.subjectExperimental glaucoma
dc.subjectMelanopsin
dc.subjectBrn3a
dc.subjectTransient ischemia of the retina
dc.subjectLong lasting neuroprotection
dc.subjectUltrasound Doppler
dc.subject.odsObjetivo 3: Salud
dc.titleMelanopsin-containing or non-melanopsin–containing retinal ganglion cells response to acute ocular hypertension with or without brain-derived neurotrophic factor neuroprotection
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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