Changes in saliva biomarkers during a standardized increasing intensity ﬁeld exercise test in endurance horses

Contreras-Aguilar, María Dolores; Cerón, J.J.; Muñoz, A.; Ayala de la Peña, Ignacio

Publication:
Changes in saliva biomarkers during a standardized increasing intensity ﬁeld exercise test in endurance horses

dc.contributor.author	Contreras-Aguilar, María Dolores
dc.contributor.author	Cerón, J.J.
dc.contributor.author	Muñoz, A.
dc.contributor.author	Ayala de la Peña, Ignacio
dc.contributor.department	Medicina y Cirugía Animal
dc.date.accessioned	2024-09-09T11:53:15Z
dc.date.available	2024-09-09T11:53:15Z
dc.date.issued	2021
dc.description	© 2021. The authors. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0 This document is the published version of a published work that appeared in final form in Animal. To access the final work, see DOI: https://doi.org/10.1016/j.animal.2021.100236	es
dc.description.abstract	Salivary biomarkers could be useful to evaluate stress, ﬁtness level, and skeletal muscle damage associ-ated to exercise in horses in an easy and non-painful way. Therefore, this study aims to evaluate if cortisol in saliva (sCor), salivary alpha-amylase (sAMY) and butyrylcholinesterase (sBChE) and lactate (sLA) and creatine kinase (sCK) in saliva of horses can show changes during a standardized exercise test, and if they are related to heart rate variability (HRV) parameters related to sympathetic and parasympathetic tone, ﬁtness level or skeletal muscle damage. For this purpose, ten endurance horses were submitted to a stan-dardized exercise test in ﬁeld conditions. Saliva and blood were obtained at basal time (TB), after the seven bouts of velocity (T + 01 to T + 07), and 5, 15, 30, and 45 min later (T + 5, T + 15, T + 30, and T+ 45). Five endurance horses in resting condition (control group) were also enrolled. HRV and ﬁtness level parameters, and plasma CK as a marker of muscle damage were also evaluated. Salivaryalpha-amylase increased at T + 30 (P = 0.03), sBChE at T + 5 (P = 008), and sCK at T + 07 (P = 0.009) after the exercise test, with signiﬁcant differences between the exercise and control groups’ results. The sCor did not show sig-niﬁcant changes during the exercise test in the exercise group but higher concentration compared to the control horses (P < 0.001) were observed. sCor, sAMY, sBChE, and sCK showed a positive correlation (r val-ues between 0.47 and 0.64) with the sympathetic tone and a negative correlation (r values between 0.37 and 0.56) with the parasympathetic tone. In conclusion, sAMY, sBChE, and sCK showed signiﬁ-cant increases in ten endurance horses after an increasing intensity velocity exercise. Values of sCor, sAMY, sBChE, and sCK were associated with HRV, which is used to evaluate stress, and therefore, they could be potentially used to assess the exercise-related stress after a physical effort.	es
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.citation	Animal 15 (2021) 100236
dc.identifier.doi	https://doi.org/10.1016/j.animal.2021.100236
dc.identifier.issn	Print: 1751-7311
dc.identifier.issn	Electronic: 1751-732X
dc.identifier.uri	http://hdl.handle.net/10201/143741
dc.language	eng	es
dc.publisher	Elsevier B.V.	es
dc.relation	This work was supported by the Seneca foundation of Murcia Regional Government, Spain (grant number 19894/GERM/15). M. D Contreras-Aguilar was granted by the predoctoral contract ‘FPU’ of University of Murcia (R- 605/2016), Spain.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S1751731121000781?via%3Dihub	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Heart rate variability	es
dc.subject	Horses	es
dc.subject	Physical effort	es
dc.subject	Salivary analytes	es
dc.subject	Stress	es
dc.title	Changes in saliva biomarkers during a standardized increasing intensity ﬁeld exercise test in endurance horses	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es