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Liquid biopsy of circulating tumor cells: From isolation, enrichment, and genome sequencing to clinical applications

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dc.contributor
dc.contributor.authorTan Keqin
dc.contributor.authorZhu Hong
dc.contributor.authorMa Xuelei
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-10-20T11:53:59Z
dc.date.available2025-10-20T11:53:59Z
dc.date.issued2025
dc.description.abstractCirculating tumor cells (CTCs), shed from primary tumors into the bloodstream, play a crucial role in metastasis and hold great potential in cancer diagnosis, prognosis, and treatment monitoring. Conventional CTC detection using epithelial biomarkers like epithelial cell adhesion molecule (EpCAM) for immunocapture overlooks mesenchymal-like CTCs with high metastatic potential, spurring the development of non-immunocapture technologies that use biophysical traits for enrichment. Innovations in microfluidic platforms and multi-parametric sorting improve isolation efficiency and address related challenges. Breakthroughs in single-cell genomic and transcriptomic sequencing enable in-depth molecular characterization of CTCs. Clinically, CTC enumeration and molecular profiling are emerging as real-time tools for assessing therapeutic response and predicting outcomes, especially in metastatic breast, prostate, and colorectal cancers. This review focuses on CTC isolation, enrichment techniques, their applications in different tumors, downstream analysis progress, and potential in precision medicine
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dc.format.extent18
dc.identifier.citationHistology and Histopathology, volúmen 40, nº 10 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-924
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/167650
dc.languageeng
dc.publisherUniversidad de Murcia, Departamento de Histología e Histopatología
dc.relationSin financiación externa a la Universidad
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCirculating tumor cells
dc.subjectIsolation
dc.subjectEnrichment
dc.subjectSequencing
dc.subjectCharacterization
dc.subjectClinical applications
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleLiquid biopsy of circulating tumor cells: From isolation, enrichment, and genome sequencing to clinical applications
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublication
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Histology and histopathology Vol.40,nº10 (2025)