Publication: Vitamin E prevents neutrophil accumulation and attenuates tissue damage in ischemic-reperfused human skeletal muscle
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Date
1997
Authors
Formigli, L. ; Ibba-Manneschi, L. ; Tani, A. ; Gandini, E. ; Adembri, C. ; Pratesi, C. ; Novelli, G.P. ; Zecchi-Orlandini, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Neutrophil accumulation and the consequent
production of oxygen-derived free radicals are involved
in the pathogenesis of Ischemia-Reperfusion syndrome.
In this study we investigated whether a treatment with
Vitamin E, which has antioxidant properties, could
attenuate the tissue damage by interfering with the influx
of neutrophils within the ischemic and reperfused human
skeletal muscle.
To this purpose, patients undergoing aortic crossclamping
during the surgical repair of aortic abdominal
aneurysm were studied as a model of ischemiareperfusion
of the lower limb muscles. Muscle biopsies
from the right femoral quadriceps of patients not
receiving and receiving Vitamin E pretreatment before
surgery were taken: a) after the induction of anaesthesia,
as control samples, and b) after a period of ischemia
followed by 30 min of reperfusion. The tissue samples
were either routinely processed for morphological study
and immunohistochemical analysis to detect an altered
expression of specific endothelial adhesion proteins,
such as E-selectin and ICAM-1. The results obtained
showed that Vitamin E administration was able to
prevent the accumulation of neutrophils within the
ischemic and reperfused muscle. This beneficia1 effect of Vitamin E was due to its ability to hinder the expression
of E-selectin and ICAM-1, molecules known to increase
the adhesiveness of endothelium to circulating
neutrophils. After treatment with Vitamin E a marked
attenuation of the reperfusion injury was also evident. In
conclusion, Vitamin E treatment may be considered a
valuable tool for protection against the ischemiareperfusion
damage of human skeletal muscle.
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