Publication:
DNA extraction from paraff in-em bedded tissues using a salting-out procedure, a reliable method for PCR amplification of archiva1 material

Simple item page
dc.contributor.authorHowe, J.R.es
dc.contributor.authorKlimstra, D.S.es
dc.contributor.authorCordon-Cardo, C.
dc.date.accessioned2011-02-22T10:41:34Z
dc.date.available2011-02-22T10:41:34Z
dc.date.issued1997
dc.description.abstractMany techniques have been described for the extraction of DNA from paraffin-embedded tissues. Numerous efforts have been directed at simplification of these methods for rapid analysis using PCR. One disadvantage to some of the simpler procedures is inefficient PCR amplification, and for more involved ones using phenol/chloroform extraction, reduction in the yield of DNA. In the present study we report the use of a novel salting-out procedure that was utilized to extract DNA from 259 separate microdissection specimens of formalin-fixed, paraffin-embedded tissue sections. These sections were derived from 97 patients with tumors of the ampulla of Vater resected between 1965 and 1995 at our institution. The mean DNA yield was 22.75 pg (median 13.2130.25) and the mean 2601280 absorbance ratio was 1.68 (median 1.7020.25). Al1 specimens (2591259) were successfully used to amplify K-ras exon 1 by a nested PCR technique. These results indicate that this DNA extraction method produces good yields of quality DNA, even from specimens several decades old.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18964
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectDNA extractiones
dc.subjectPCR techniquees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleDNA extraction from paraff in-em bedded tissues using a salting-out procedure, a reliable method for PCR amplification of archiva1 materiales
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
DNA extraction from paraff inem bedded tissues using a saltingout procedure a reliable method for PCR amplification of archiva1 material.pdf
Size:
1.01 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.12, nº 3 (1997)