In vitro evaluation of cell death induced by cadmium, lead and their binary mixtures on erythrocytes of Common buzzard (Buteo buteo)

Hernández-García, A.; Romero, D.; María-Mojica, P.; Martínez-López, E.; García Fernández, Antonio Juan; Gómez Ramírez, Pilar

Publication:
In vitro evaluation of cell death induced by cadmium, lead and their binary mixtures on erythrocytes of Common buzzard (Buteo buteo)

dc.contributor.author	Hernández-García, A.
dc.contributor.author	Romero, D.
dc.contributor.author	María-Mojica, P.
dc.contributor.author	Martínez-López, E.
dc.contributor.author	García Fernández, Antonio Juan
dc.contributor.author	Gómez Ramírez, Pilar
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2024-03-08T12:36:41Z
dc.date.available	2024-03-08T12:36:41Z
dc.date.issued	2013-11-25
dc.description	©2013 Elsevier. This document is the Published version of a Published Work that appeared in final form in Toxicology in Vitro. To access the final edited and published work see https://doi.org/10.1016/j.tiv.2013.11.005	es
dc.description.abstract	Cadmium and lead are persistent and ubiquitous metals that can cause several deleterious effects in living beings. Apoptosis and necrosis are two types of cell death that can be found after in vivo and in vitro exposure to these metals. In this study, isolated red blood cells from living captive Common buzzard (Buteo buteo) were exposed in vitro to different concentrations of lead, cadmium, and the mixture lead–cadmium in a proportion of 1:10 (similar to that found in previous field studies). Data obtained from dose–response curves were used to evaluate the interactive effects of metal mixtures on cell viability. In general, except for the exposure to NOEC, additivity was the most frequently observed response. As described in human, after in vitro exposure, lead was highly accumulated in buzzard erythrocytes, while cadmium accumulation was scarce. Finally, the type of cell death (apoptosis or necrosis) induced by the exposure to different concentrations of these heavy metals and their mixtures was evaluated in the red blood cells. Apoptosis was found to be the main type of cell death observed after cadmium and/or lead exposure. However, this exposure caused an increase in lysis or necrosis, especially if red blood cells were exposed to high doses.	es
dc.format	application/pdf	es
dc.format.extent	7	es
dc.identifier.citation	Toxicology in Vitro, Vol. 28, N. 2, 2014, pp. 300-306
dc.identifier.doi	https://doi.org/10.1016/j.tiv.2013.11.005
dc.identifier.issn	Print: 0887-2333
dc.identifier.uri	http://hdl.handle.net/10201/140013
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Special thanks to Generalitat Valenciana for permission to obtain the samples. Thanks also to the Ministry for Science and Innovation for their financial support via the NOVEDAR (Consolider-Ingenio 2010), and MASCA’2008 (CGL-2008-4318/BOS) projects. Also thanks to Seneca Foundation of the Autonomous Community of Murica Region (CARM) for the 08758/PI/08 Project.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0887233313003093?via%3Dihub	es
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Binary mixtures	es
dc.subject	Cell death	es
dc.subject	Common buzzard	es
dc.subject	Erythrocytes	es
dc.subject	Apoptosis	es
dc.subject	Buteo buteo	es
dc.title	In vitro evaluation of cell death induced by cadmium, lead and their binary mixtures on erythrocytes of Common buzzard (Buteo buteo)	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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