Publication: Elucidation of soft tissue flap histologic
margins within a canine vocal fold
Authors
Glab, Rachel C. ; Gunderson, McLean ; Torrealba, José ; Bauer, Ben ; Dailey, Seth H.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-30.95
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info:eu-repo/semantics/article
Description
Abstract
Summary. Background: Histologic identification of
implanted soft tissues in experimental animal models
can be challenging, as donor tissue often strongly
resembles the recipient bed. We have encountered this
dilemma following implantation of a Composite Thyroid
Ala Perichondrium flap (CTAP) into a vocal fold. The
CTAP procedure is the first to utilize a vascularized flap
for vocal fold reconstruction, making data to confirm or
refute its viability critical. The current study evaluated
several tissue stains to define precisely the histologic
margins of CTAPs at two weeks post-implantation in a
canine model.
Methods: Initial testing exposed canine cadaveric
tissues to four stains (tattoo ink, Congo red, 4’6-
diamidino-2-phenylindole, and henna) across four time
periods. Tattoo ink alone withstood histologic
processing. An exposure of 1 minute adequately
delineated CTAP boundaries. The study concluded with
a canine in vivo evaluation of a CTAP exposed to tattoo
ink for 1 minute. After a two-week recovery period,
vocal folds were harvested and evaluated histologically.
Results: Tattoo ink proved to be a safe and effective
histologic marker in vivo, where the histologic margins
of the implanted CTAP were clearly demarcated by a
thin band of tattoo ink, soft tissue reactions were
minimal, and interference with standard, special, or
immunohistochemical stain assessments did not occur.
Conclusions: Tattoo ink provides a reliable means of
demarcating a CTAP within a vocal fold and
demonstrated that CTAPs survive transplantation.
Further, tattoo ink demarcation may serve as a useful
histologic marker for those wishing to assess tissue
implants in other in vivo models.
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Citation
Histology and Histopathology, Vol. 30, n.º 1 (2015)
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