Publication: Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor
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Date
2008-04-07
Authors
Mulero Méndez, Iván ; Meseguer Peñalver, J. ; García Ayala, Alfonsa ; Mulero Méndez, Victoriano Francisco ; Sepulcre Cortés, María Pilar ; Roca Soler, Francisco José
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.dci.2008.03.005
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info:eu-repo/semantics/article
Description
© 2008 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Developmental and Comparative Immunology. To access the final edited and published work see https://doi.org/10.1016/j.dci.2008.03.005
Abstract
Two major professional phagocyte populations have been described in fish, namely granulocytes and monocytes/macrophages. Although the distribution and localization of macrophages have been documented in several teleost species using mainly light and/or electron microscopy, the lack of appropriate markers for these cells has hampered our in-depth knowledge of their biology. We report here the generation of a monospecific rabbit polyclonal antibody against the gilthead seabream macrophage colony-stimulating factor receptor (Mcsfr), which is an excellent marker of macrophages in mammals and the zebrafish. The anti-Mcsfr has been found to be very useful in immunohistochemistry (IHC) to specifically immunostain the purified macrophages (adherent cells) obtained from the head-kidney as well as different cell populations in paraffin-embedded organs, including the head-kidney, spleen, thymus, gills and liver. Unexpectedly, however, no Mcsfr immunoreactive (Mcsfr+) cells were observed in the brain and intestine of the gilthead seabream. We also show that the distribution of Mcsfr+ cells in the head-kidney and the spleen is unaltered following infection with the fish pathogenic bacterium Vibrio anguillarum and that the Il1b-producing cells in these two organs after infection are exclusively acidophilic granulocytes. Finally, as the epitope recognized by the anti-Mcsfr is well conserved, we illustrate the potential usefulness of this antibody in other teleost species, such as the European seabass.
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Citation
Developmental and Comparative Immunology (2008) 32, 1151–1159
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