Publication: Phenotypic changes and possible angiogenic roles
of pericytes during wound healing in the mouse skin
Authors
Morikawa, Shunichi ; Ezaki, Taichi
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Publisher
Editores F. Hernan dez y Juan F. Madrid. Murcia, Universidad de Murcia, Departamento de Biologia Celular e Histologia
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Pericytes (PCs) are attracting increasing
attention as a crucial target for anti-angiogenic therapy.
In this study, we sought to determine the functional
significance of PCs during angiogenesis by using a skin
wound healing model in which different angiogenic
stages are identifiable. Angiogenesis was first observed
on Day 3 after wounding and increased greatly on Day
5. On Day 5, the leading edge of the regenerating vessels
(vascular advancing front; VAF) appeared to be
composed of immature vessels, and was further divided
into “tip” and “following” regions according to
maturational differences. PCs distributed in regenerating
vessels showed phenotypic differences according to
different regions. PCs that expressed PDGFR-ß alone
and lacked vascular basement membrane (BM) were
predominant in the tip region of the VAF, while PCs that
expressed both PDGFR-ß and NG2 with their BM
coating were numerous in the following regions toward
the rear of the VAF. Moreover, PCs in the VAF
expressed VEGF-A and associated with most
proliferating endothelial cells (ECs). VEGF-A
expression of PCs and the proliferating ECs totally
disappeared in the region toward the rear of the VAF. We
conclude that PCs can differ in their phenotype
according to the stage of angiogenesis during wound
healing. They may promote angiogenesis at the initial
stage but might in turn stabilize the newly formed
vessels at the later stage.
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