Publication: Implications on pathogenesis and risk of oral lichen planus neoplastic transformation: an ex-vivo retrospective immunohistochemical study
Authors
Squarzanti, Diletta Francesca ; Cena, Tiziana ; Sorrentino, Rita ; Migliario, Mario ; Chiocchetti, Annalisa ; Rimondini, Lia ; Azzimonti, Barbara ; Valente, Guido
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
DOI: 10.14670/HH-18-104
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info:eu-repo/semantics/article
Description
Abstract
Aims. To evaluate OPN, MCM7, Ki-67, p53,
Bcl-2 and 53BP1 presence, together with the abnormal
adaptive CD4 and CD8 T-cell response markers
expression in a series of oral lichen planus (OLP)
affected patients and assess their combined contribution
for a more objective disease classification.
Methods and results. In this ex-vivo retrospective
analysis, biopsy specimens from 28 adults with a clinical
diagnosis of OLP at different progression degree (16
reticular, 2 plaque-like, 1 erosive and 9 mixed type) were
collected. Sections were immunohistochemically
investigated for the proinflammatory cytokine
osteopontin (OPN), alpha-beta CD4 and CD8 positive T
cells, DNA replication licensing factor (MCM7),
proliferating cell marker (Ki-67), apoptotic and tumor
antigen (p53), apoptosis modulator (Bcl-2) and cellular
response regulator to double-strand breaks tumor
suppressor p53-binding protein 1 expression.
Statistical analysis revealed that 53BP1 is highly
represented among the OLP study patients (p<0.05).
Moreover, on the basis of the quantification results of
the highly expressed parameters, two illness categories
with different severity were evidenced. The
classification hypothesis was confirmed by i) OLP lesion
persistence, ii) the development of oral severe lesions in
the patients belonging to high grade activity OLP group
(HGA-OLPs) and iii) the ascertainment of the same
evidence both in the oral squamous cell tumor controls
(OSCC) and in HGA-OLP cases.
Conclusion. This study completes the scenario with
respect to early detection, thanks to a more precise
histological analysis, for rationalizing the clinical and
histological findings toward a sharable international
disease scoring system.
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Citation
Histology and Histopathology, Vol.34, nÂş9, (2019)
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