Publication: The molecular determinants of the efficiency of green fluorescent protein mutants
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Date
2000
Authors
Sacchetti, A. ; Ciccocioppo, R. ; Alberti, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The Green Fluorescent Protein (GFP) is a
spontaneously fluorescent polypeptide of 27 kD from the
jellyfish Aequorea victoria that absorbs UV-blue light
and emits in the green region of the spectrum. GFP has
been succesfully expressed both in bacteria and in
eukaryotic cells and is widely used to monitor the
localization of tagged proteins in living cells. Since
wtGFP performs inefficiently in different cellular
contexts, efforts have been devoted to the improvement
of GFP expression levels andtor fluorescence. We will
here review the basic characteristics of wt and mutated
GFP, in particular their protein expression vs fluorescent
properties. Emphasis will be given to unexpected
consequences of mutations of the GFP gene, i.e. on
transcription and translation rates and on protein folding
in different cell types, and to how these critically reflect
on the use of GFP in different cellular environments.
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