Publication: lntermediate filament protein expression and sugar moieties in normal canine placenta
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Date
2000
Authors
Fernandez, P.E. ; Barbeito, C.G. ; Portiansky, E.L. ; Gimeno, E.J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In the female dog, the placenta is considered
zonal, endotheliochorial and labyrinthic. The distribution
of the intermediate filaments as well as the surface
glycoproteins in the canine placenta are still unknown.
The aim of the present study was to provide this
information for further understanding of pathological
conditions in the bitch. Samples were obtained from
normal uterine horns at the end of gestation. Tissues
were routinely fixed and stained. Monoclonal antibodies
against cytokeratins, vimentin and desmin were used for
immunohistochemical staining. UEA-1, PNA, RCA-1,
SBA, DBA, WGA and ConA were used for the lectin
histochemical staining. A computer morphometrical
analysis was made. Statistical analysis was then
accomplished. The results showed the maximun
immunohistochemical percentage for vimentin in the
supraglandular connective tissue, for pancytokeratin in
the spongy zone and for desmin in miometrium. SBA
showed the highest staining percentage in the gland cells
of the spongy zone, while ConA was the highest in the
syncytiotrophoblastic cells and gland cells of the deep
glandular zone. The results obtained indicate that the
lectin binding pattern is partially different from other
animal species. On the contrary, the intermediate
filament data coincide with similar observations from
other mammals.
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