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A systematic review and meta-analysis of the validation of serological methods for detecting anti-Toxoplasma gondii antibodies in humans and animals

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dc.contributor.advisorFacultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
dc.contributor.authorHuertas López, Ana
dc.contributor.authorCantos Barreda, Ana
dc.contributor.authorSánchez Sánchez, Roberto
dc.contributor.authorMartínez-Carrasco Pleite, Carlos
dc.contributor.authorMartínez Subiela, Silvia
dc.contributor.authorCerón Madrigal, José Joaquín
dc.contributor.authorIbáñez López, Francisco Javier
dc.contributor.authorÁlvarez García, Gema
dc.contributor.departmentSanidad Animal
dc.coverage.temporal2013-2023
dc.date.accessioned2024-04-22T07:25:14Z
dc.date.available2024-04-22T07:25:14Z
dc.date.issued2024-03-21
dc.description©2024 Elsevier. This document is the Published version of a Published Work that appeared in final form in Veterinary Parasitology. To access the final edited and published work see https://doi.org/10.1016/j.vetpar.2024.110173
dc.description.abstractToxoplasma gondii is a paradigmatic zoonotic parasite from the One Health perspective, since it is broadly distributed and virtually infects all warm-blooded species. A wide variety of serological techniques have been developed to detect T. gondii infection in humans and animals. Our aim was to describe and compare the main characteristics of these serological tests and validation processes and to critically analyze whether these tests meet the standards required to ensure an accurate serological diagnosis. The current systematic review and metaanalysis included 134 studies that were published from 2013 to 2023. QUADAS 2 tool was used to evaluate the quality of the included studies. A total of 52 variables related to the characteristics of the techniques and analytical and diagnostic validation parameters were studied. A wider panel of tests was developed for humans, including techniques exclusively developed for humans that involve costly equipment and the measurement of different Ig isotypes that are considered biomarkers of congenital toxoplasmosis. Studies conducted in humans frequently employed commercial techniques as reference tests, measured different immunoglobulin isotypes with a predominance for IgG (>50%) and discriminated between acute and chronic infections. In animals, the most commonly used reference techniques were in-house tests, which almost exclusively detected IgG. Common limitations identified in a large number of studies were some misunderstandings of the terms “gold standard” and “reference test” and the absence of information about the negative and positive control sera used or the exact cutoff employed, which were independent of the quality of the study. There is a lack of analytical validation, with few evaluations of cross-reactivity with other pathogens. Diagnostic odds ratio values showed that indirect ELISA based on native or chimeric antigens performed better than other tests. The reproducibility of serological test results in both humans and animals is not guaranteed due to a lack of relevant information and analytical validation. Thus, several key issues should be considered in the future, including interlaboratory ring trials.eng
dc.formatapplication/pdf
dc.format.extent17
dc.identifier.doihttps://doi.org/10.1016/j.vetpar.2024.110173
dc.identifier.eisbnVeterinary Parasitology, 328 (2024) 110173eng
dc.identifier.issnPrint: 0304-4017
dc.identifier.urihttp://hdl.handle.net/10201/141039
dc.languageengeng
dc.publisherElsevier
dc.relationAna Huertas Lopez was supported by a predoctoral grant from the University of Murcia [grant number R-1207/2017] and by a postdoctoral contract Margarita Salas (University of Murcia) from the Program of Requalification of the Spanish University System (Spanish Ministry of Universities) financed by the European Union — NextGenerationEU [grant number R-1593/2022]; Gema ´Alvarez García is part of the TOXOSOURCES consortium, supported by funding from the European Union’s Horizon 2020 Research and Innovation Programme [grant agreement no. 773830: One Health European Joint Programme]; Ana Cantos Barreda was supported by a contract co-financed 91.89% by the European Social Fund and the “Iniciativa de Empleo Juvenil (POEJ)” through the Seneca Foundation of Murcia Region, Spain [grant number 21327/PDGI/19]; finally, this work was supported by the Seneca Foundation of Murcia Region, Spain [project 19894/GERM/15].es
dc.relationSin financiación externa a la Universidad
dc.relation.publisherversionhttps://www.journals.elsevier.com/veterinary-parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccesses
dc.subjectMeta-analysiseng
dc.subjectOne Healtheng
dc.subjectSerological techniqueseng
dc.subjectToxoplasma gondiieng
dc.subjectValidationeng
dc.subject.otherCDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleA systematic review and meta-analysis of the validation of serological methods for detecting anti-Toxoplasma gondii antibodies in humans and animalseng
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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