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Season of ejaculate collection influences the freezability of boar spermatozoa

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dc.contributor.authorBarranco Cascales, Isabel
dc.contributor.authorOrtega, María D.
dc.contributor.authorMartínez Alborcia, María J.
dc.contributor.authorVázquez, Juan M.
dc.contributor.authorMartínez García, Emilio
dc.contributor.authorRoca Aleu, Jorge
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2025-10-16T12:54:54Z
dc.date.available2025-10-16T12:54:54Z
dc.date.copyright© 2013 Elsevier Inc. All rights reserved
dc.date.issued2013-12-03
dc.description.abstractThe aim of this retrospective study was to evaluate whether the season of ejaculate collection influences the freezability of porcine sperm. A total of 434 ejaculates were collected from boars of six different breeds over three years (2008-2011) and throughout the four seasons of the year identified in the northern hemisphere (winter, spring, summer and autumn). The ejaculates were cryopreserved using a standard 0.5 mL straw freezing protocol. Sperm quality was assessed before (fresh semen samples kept 24h at 17°C) and after freezing and thawing (at 30 and 150 min post-thawing in semen samples kept in a water bath at 37 °C), according to the percentages of total motility, as assessed by the CASA system, and viability, as assessed by flow cytometry after staining with SYBR-14, PI and PE-PNA. The data, in percentages, on sperm motility and viability after freezing and thawing were obtained at each evaluation time (recovered) and were normalized to the values before freezing (normalized). The season of ejaculate collection influenced (P<0.01) sperm quality before freezing and after thawing (recovered and normalized), irrespective of the breed of boar. Sperm quality was lower in summer, both in terms of motility and viability, and in autumn, in terms of motility, than in winter and spring. Seasonality in the normalized data indicates that the season of ejaculate collection influences sperm freezability, regardless of the season's influence on sperm quality before freezing. Consequently, the spermatozoa from ejaculates collected during summer and, to a lesser extent, also in autumn, are more sensitive to cryopreservation than those from ejaculates collected during winter and spring.
dc.formatapplication/pdf
dc.format.extent6
dc.identifier.citationCryobiology, 2013, Vol. 67, Issue 3, pp. 299-304
dc.identifier.doihttps://doi.org/10.1016/j.cryobiol.2013.09.001
dc.identifier.eissn0011-2240
dc.identifier.urihttp://hdl.handle.net/10201/167089
dc.languageeng
dc.publisherElsevier
dc.relationThe authors are grateful to AIM Iberica and Hypor España for supplying the boar ejaculates. This work was supported by GERM (04543/07), Murcia (Spain); MINECO (AGL2012-39903), Madrid (Spain) and FEDER funds.
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0011224013002204
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCryopreservation
dc.subjectPorcine
dc.subjectSeasonality
dc.subjectSperm
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleSeason of ejaculate collection influences the freezability of boar spermatozoa
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
relation.isAuthorOfPublication54a701a0-5cff-4dde-8614-de81f8c2ce55
relation.isAuthorOfPublication032b17bf-c6a7-4745-a513-c02b384cc26c
relation.isAuthorOfPublication.latestForDiscovery3c34d0d5-7d7d-4bcc-a119-8906f53baa7b
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