Diversity of symbiotic cyanobacteria in cycad coralloid roots using a short-read rbcL-X amplicon

Sierra, Adriel M.; Toupin, Sandrine; Villarreal A., Juan Carlos; Alonso García, Marta

Publication:
Diversity of symbiotic cyanobacteria in cycad coralloid roots using a short-read rbcL-X amplicon

dc.contributor.author	Sierra, Adriel M.
dc.contributor.author	Toupin, Sandrine
dc.contributor.author	Villarreal A., Juan Carlos
dc.contributor.author	Alonso García, Marta
dc.contributor.department	Biología Vegetal
dc.date.accessioned	2025-01-17T11:57:36Z
dc.date.available	2025-01-17T11:57:36Z
dc.date.issued	2024-02-21
dc.description	© 2024. The Author(s), under exclusive licence to Springer Nature B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This document is Accepted version of a Published Work that appeared in final form in Symbiosis. To access the final edited and published work see https://doi.org/10.1007/s13199-024-00972-w
dc.description.abstract	Cycads are the only gymnosperms forming a symbiosis with nitrogen-fixing cyanobacteria in a specialized organ: the coralloid root. This paper investigates the endophytic bacterial community inhabiting the coralloid roots of two cycads from Panama. We sampled coralloid roots from Zamia nana (terrestrial) and Zamia pseudoparasitica (epiphytic). Then, we used the 16S rRNA amplicon marker to describe the entire bacterial community. We also designed a new marker to amplify the rbcL-rbcX spacer and around 100 bp of the rbcX gene, targeting cyanobacteria. We found that using 16S, endophytic bacteria diversity is represented mainly by the phyla Actinobacteria, Cyanobacteria, and Proteobacteria. In addition, 16S analyses showed that Zamia species do not share a core cyanobacterial community (using stringent 75% and 90% thresholds), while the two species shared 4 ASVs at a 50% threshold. The newly developed rbcL-rbcX marker revealed that both species share a core cyanobacterial community represented by a single amplicon sequence variant (ASV1) (Nostoc sp.) at 90% threshold that is found in the same phylogenetic clade of that contain mostly Panamanian symbiotic cyanobacteria. Using a 75% threshold, only three ASVs (ASV1, ASV2, ASV3) were present across samples, and five ASVs at 50% threshold. This new marker can effectively identify cyanobacteria ASVs and provide a better resolution for microbial analyses in autotroph cyanobacterial symbioses.	es
dc.format	application/pdf	es
dc.format.extent	19	es
dc.identifier.citation	Symbiosis 92, 271–288 (2024)
dc.identifier.doi	https://doi.org/10.1007/s13199-024-00972-w
dc.identifier.issn	Print: 0334-5114
dc.identifier.issn	Electronic: 1878-7665
dc.identifier.uri	http://hdl.handle.net/10201/148704
dc.language	eng	es
dc.publisher	Springer Nature	es
dc.relation	The program Établissement de nouveaux chercheurs universitaires—FRQNT-206943 (Québec) and the Research Chair of Canada on the genomics of the symbiosis between tropical plants and microbes; Canada Foundation for Innovation #36,781, #39,135; Earl S. Tupper Fellowship (STRI); Simons Foundation to the Smithsonian Tropical Research Institute #429,440.	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s13199-024-00972-w	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Zamia	es
dc.subject	16S rRNA marker
dc.subject	New marker
dc.subject	Core cyanobacterial community
dc.subject	Panama
dc.title	Diversity of symbiotic cyanobacteria in cycad coralloid roots using a short-read rbcL-X amplicon	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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relation.isAuthorOfPublication.latestForDiscovery	b87695f3-6eea-41fa-9863-958f9b63e2b2