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Differentiation of human mesenchymal stromal cells cultured on collagen sponges for cartilage repair

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dc.contributor.authorSanjurjo Rodríguez, Clara
dc.contributor.authorMartínez Sánchez, Adela Helvia
dc.contributor.authorHermida Gómez, Tamara
dc.contributor.authorFuentes Boquete, Isaac
dc.contributor.authorDíaz Prado, Silvia
dc.contributor.authorBlanco, Francisco J.
dc.date.accessioned2021-11-16T10:28:55Z
dc.date.available2021-11-16T10:28:55Z
dc.date.issued2016
dc.description.abstracty. Aim: The aim of this study was to evaluate proliferation and chondrogenic differentiation of human bone-marrow mesenchymal stromal cells (hBMSCs) cultured on collagen biomaterials. Materials and Methods: hBMSCs were seeded on five different collagen (Col) sponges: C1C2 (types I and II Col), C1C2HS (types I and II Col plus heparan sulphate (HS)), C1C2CHS (types I and II Col plus chondroitin sulphate (CHS)), C1-OLH3 (type I Col plus low molecular weight heparin) and C1CHS (type I Col plus CHS). The resulting constructs were analyzed by histological and immunohistochemical staining, molecular biology and electron microscopy. Col released into culture media was measured by a dye-binding method. Results: hBMSCs on biomaterials C1C2, C1C2HS and C1C2CHS had more capacity to attach, proliferate and synthesize Col II and proteoglycans in the extracellular matrix (ECM) than on C1-OLH3 and C1CHS. The presence of aggrecan was detected only at the gene level. Total Col liberated by the cells in the supernatants in all scaffold cultures was detected. The level of Col I in the ECM was lower in C1-OLH3 and that of Col II was highest in C1C2 and C1C2HS. Electron microscopy showed differently shaped cells, from rounded to flattened, in all constructs. Col fibers in bundles were observed in C1C2CHS by transmission electron microscopy. Conclusions: The results show that Col I and Col II (C1C2, C1C2HS and C1C2CHS) biomaterials allowed cell proliferation and chondrogenic-like differentiation of hBMSCs at an early stage. Constructs cultured on C1C2HS and C1C2CHS showed better cartilage-like phenotype than the other ones.es
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dc.format.extent19es
dc.identifier.citationHistology and Histopathology, Vol.31, nº11, (2016)
dc.identifier.doiDOI: 10.14670/HH-11-754
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/114045
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTissue Engineeringes
dc.subjectOsteoarthritises
dc.subjectMesenchymal Stromal Cellses
dc.subjectBiomaterialses
dc.subjectChondrogenesises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleDifferentiation of human mesenchymal stromal cells cultured on collagen sponges for cartilage repaires
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.31,nº 11 (2016)