Publication: Isolation of high quality protein samples from punches of formalin fixed and paraffin embedded tissue blocks
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Date
2008
Authors
Kroll, J. ; Becker, K.F. ; Kuphal, S. ; Hein, R. ; Hofstädter, F. ; Bosserhoff, A.K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In general, it is believed that the extraction of
proteins from formalin-fixed paraffin embedded samples
is not feasible. However, recently a new technique was
developed, presenting the extraction of non-degraded,
full length proteins from formalin fixed tissues, usable
for western blotting and protein arrays. In the study
presented here, we applied this technique to punch
biopsies of formalin fixed tissues embedded in paraffin
to reduce heterogeneity of the tissue represented in
sections, and to ensure analysing mainly defined cellular
material. Successful extraction was achieved even from
very small samples (0.7 mm3). Additionally, we were
able to detect highly glycosylated proteins and protein
modification, such as phosphorylation. Interestingly,
with this technique it is feasible to extract high quality
proteins from 14 year old samples. In summary, the new
technique makes a great pool of material now usable for
molecular analysis with high throughput tools.
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