Publication: Comparative in vivo and in vitro models to approach the cellular basis of endotoxic shock. The role of sinusoidal liver cells
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Date
1996
Authors
Pagani, R. ; Portolés, M.T. ; Arahuetes, Rosa María ; Ainaga, M.J. ; Machín, Celia ; Rúa, Carmen
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
During endotoxic shock, the liver exerts a
lipopolysaccharide (LPS) clearance function with the
participation of both parenchymal and sinusoidal cells.
Liver damage could be caused by LPS direct action,
hypoxia and/or inflammatory mediators released by
Kupffer cells.
The aim of this study is to establish an experimental
model that could allow us to understand the direct E. coli
O1 1 l:B4 LPS action on sinusoidal cells. A comparative
study was carried out, in vivo and in vitro, using either a
rat reversible endotoxic shock model or sinusoidal cell
cultures.
The LPS was found to induce important and similar
morphological alterations both in vivo and in vitro,
specially in Kupffer cells. These cells present
mitochondrial damage, nuclear membrane swelling, and
increased number of phagosomes, including lamellar
bodies. An immunocolloidal gold technique shows, in
vitro, the LPS mainly located on Kupffer cell membrane
and in phagosomes. The LPS binding to membrane, as a
primary step of Kupffer cell activation, increases the
phagocytosis. This effect could be related to a decrease
of fluidity on the externa1 membrane portion.
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