Publication: Argentic staining reveals changes in
cerebellar tissue organisation by prenatal
glucocorticoid administration in rats
Authors
Rivas-Manzano, Patricia ; Ramírez-Escoto, María Marcela ; De la Rosa-Rugerio, Concepción ; Rugerio-Vargas, Concepción ; Ortiz-Hernández, Rosario ; Torres-Ramírez, Nayeli
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-291
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info:eu-repo/semantics/article
Description
Abstract
It was almost 150 years ago that Golgi
revolutionised histology with silver-based stains. Major
advances in knowledge of the nervous system became
possible because of silver impregnations. Silver staining
combined with classical histological staining,
cytochemistry methods, and electron microscopy is
useful for studying mechanisms and components at
subcellular, cellular, and tissue levels. Despite the
advantages of silver staining, its use has decreased over
time. The aim of this work was to use argentic staining
to study the cerebellar effects of controversial prenatal
glucocorticoid (GC) therapy. At postnatal day 12 (P12),
the cerebellum of corticosterone (CC)-treated rats
impregnated with AgNOR staining exhibited diminished
thickness of the external granule layer (EGL) and
irregular Purkinje cell arrangement. There was a greater
number of nucleoli and nucleolar organiser regions
(NORs) in 24% of Purkinje cells. Cerebellar granule
neuron progenitor (CGNP) cells of the EGL showed a
decrease in cellular density (confirmed by proliferating
cell nuclear antigen [PCNA] immunolocalization) and
NORs. At postnatal day 6 (P6), the Golgi-Kopsch
technique allowed us to observe disturbances in the
distribution pattern of CGNP cells (during proliferation,
migration, and differentiation) and premature growth of
the Bergmann glia. Our findings reveal disturbances in
the cerebellar development program with early cellular
and tissue changes.
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Citation
Histology and Histopathology Vol. 36, nº2 (2021)
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