Publication: Some morphological and histochemical features
of the midgut myenteric plexus of the common
european frog, Rana Esculenta
Authors
Gabriel, Robert ; Fekete, Eva ; Csoknya, Maria
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The neuron morphology and distribution
of four putative transmitters were investigated in the
myenteric plexus of frog (Rana esculents) midgut. The
gross morphology was revealed by NADH-diaphorase
histochemistry, and the shape of the neurons by
silver impregnation. Nerve cells had heterogeneous
distribution: they either formed ganglia or placed as
solitary neurons in the duodenum, while in the rest of
the midgut only solitary neurons were observed. Three
morphologically distinct cell types were rcvealed by
silver impregnation: mainly type I and type I1 neurons
cells were seen in the duodenum, while the rest of
the intestine contained type I1 and I11 cells.
Catecholamine fluorescence was revealed in nerve
fibres in the duodenum, while few small nerve cells
were observed in the small intestinal region.
Acetylcholinesterase histochemistry showed strongly
reactive nerve cells that were associated with the main
fibre bundles in the duodenum. Only longitudinally
oriented fibres and occasionally stained neurons
were seen in the small intestine. Substance P
immunocytochemistry revealed an extensive plexus,
which contained a moderate number of stained
perikarya in the full length of the midgut. Gammaaminobutyric
acid showed non-uniform distribution in
the two parts of the midgut: a stronger and more
regular fibre staining was found in the duodenum then
in the rest of the intestine. Ultrastructural
observations demonstrated that intrinsic neurons
received synaptic inputs from the profiles contained
agranular vesicles. while ~PD- t y pper ofiles established
close contacts with neurons. Both profile types formed
close contacts with the smooth muscle cells. In spite of
the several neuromorphological and ultrastructural
similarities to the mammalian species, the transmitter
distribution described here does not match in all
respects to that found in the enteric nervous system of
Offprint requests to: Dr. E. Fekete, Jate, Department of Zoology,
Szeged, Egyetem U. 2., P.O. Box. 659, H-6722, Hungary
those animals. This finding reflects the view, that the
neurochemical coding system and the projection
pattern within the plexus may vary considerably
among different classes of vertebrates.
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