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Madrid Mateo, María Isabel

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Madrid Mateo, María Isabel
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Universidad de Murcia. Departamento de Genética y Microbiología
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    Metabolic control of cytokinesis by glucose cAMP–PKA signaling in fission yeast
    (Elsevier, 2025-09-19) Marín Castillo, Antonio; León Zaragoza, Sergio; Franco Sánchez, Alejandro; Vicente Soler, Jerónima; Núñez Hernández, Andrés; Soto Pino, Teresa; Madrid Mateo, María Isabel; Cansado Vizoso, José; Genética y Microbiología; Facultades de la UMU::Facultad de Biología
    Cytokinesis, the final step of cell division, must be precisely coordinated with the cellular metabolic status, yet the underlying regulatory mechanisms remain poorly understood. Here we show that in Schizosaccharomyces pombe, glucose signaling promotes cytokinesis via the evolutionarily conserved cAMP–PKA signaling pathway. Loss of the Pka1 catalytic subunit delays assembly and constriction of the contractile actomyosin ring (CAR), whereas constitutive PKA activation enhances CAR integrity and accelerates cytokinesis. Mechanistically, Pka1 downregulates the basal activity of the stress-activated MAPK Sty1 under glucose-rich conditions, thereby stabilizing the formin For3 and its nucleated actin cables, which collaborate to regulate CAR dynamics. Remarkably, cAMP–PKA signaling also facilitates cytokinesis through a parallel, actin cable–independent mechanism. Additionally, mitochondrial respiration contributes to cytokinesis in the presence of glucose through a PKA-independent pathway. These findings reveal a multilayered network that links carbon source metabolism to cytoskeletal organization and underscore the importance of tight PKA activity control for robust cell division.
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    Specific functional features of the cell integrity MAP Kinase pathway in the dimorphic fission yeast Schizosaccharomyces japonicus
    (MDPI, 2021-06-14) Gómez Gil, Elisa; Franco Sánchez, Alejandro; Vázquez Marín, Beatriz; Prieto Ruiz, Francisco; Pérez Díaz, Armando Jesús; Vicente Soler, Jerónima; Madrid Mateo, María Isabel; Soto Pino, Teresa; Cansado Vizoso, José; Genética y Microbiología; Facultades de la UMU::Facultad de Biología
    Mitogen activated protein kinase (MAPK) signaling pathways execute essential functions in eu-karyotic organisms by transducing extracellular stimuli into adaptive cellular responses. In the fis-sion yeast model Schizosaccharomyces pombe the cell integrity pathway (CIP) and its core effector, MAPK Pmk1, play a key role during regulation of cell integrity, cytokinesis, and ionic homeostasis. Schizosaccharomyces japonicus, another fission yeast species, shows remarkable differences with re-spect to S. pombe, including a robust yeast to hyphae dimorphism in response to environmental changes. We show that the CIP MAPK module architecture and its upstream regulators, PKC orthologs Pck1 and Pck2, are conserved in both fission yeast species. However, some of S. pombe’s CIP-related functions, such as cytokinetic control and response to glucose availability, are regulated differently in S. japonicus. Moreover, Pck1 and Pck2 antagonistically regulate S. japonicus hyphal differentiation through fine-tuning of Pmk1 activity. Chimeric MAPK-swapping experiments re-vealed that S. japonicus Pmk1 is fully functional in S. pombe, whereas S. pombe Pmk1 shows a limited ability to execute CIP functions and promote S. japonicus mycelial development. Our findings also suggest that a modified N-lobe domain secondary structure within S. japonicus Pmk1 has a major influence on the CIP signaling features of this evolutionarily diverged fission yeast.2021
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