Gallego Jara, Julia

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Gallego Jara, Julia

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Gallego Jara, Julia

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Universidad de Murcia. Departamento de Bioquímica y Biología Molecular"B" e Inmunología

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Open Access
The protein acetyltransferase PatZ from Escherichia coli is regulated by autoacetylation-induced oligomerization
(2015) Castaño Cerezo, Sara; Bernal Sanchez, Vicente; Manjón Rubio, Antonio; Fernández Espín, Vanesa; García de la Torre, José; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Bioquímica y Biología Molecular B e Inmunología
Background: PatZ is the main Escherichia coli acetyltransferase and control acetyl-CoA synthetase (Acs) activity. Results: The kinetic and structural PatZ oligomer characteristics were determined. Conclusion: PatZ is a stable tetramer and forms an active octamer by autoacetylation to increase its stability. Significance: PTMs by acetylation have structural and functional roles in the cell.
Open Access
Relationship between lung function and exhaled volatile organic compounds in healthy infants
(Wiley-Blackwell, 2022-01-29) Sola‐Martínez, Rosa A.; Sánchez-Solís de Querol, Manuel; Lozano Terol, Gema; Gallego Jara, Julia; García-Marcos Álvarez, Luis Vicente; Cánovas Díaz, Manuel; Diego Puente, Teresa de; NELA Study Group; Cirugía, Pediatría y Obstetricia y Ginecología; Facultad de Medicina
Objective: The aim of this study is to assess, for the first time, the relationship between the volatilome and lung function in healthy infants, which may be of help for the early detection of certain respiratory diseases. Lung function tests are crucial in chronic respiratory diseases diagnosis. Moreover, volatile organic compounds (VOCs) analysis in exhaled breath is a noninvasive technique that enables the monitorization of oxidative stress, typical of some forms of airway inflammation. Methods: Lung function was studied in 50 healthy infants of 3–8 months of age and the following parameters were obtained: forced vital capacity (FVC), forced ex piratory volume at 0.5 s (FEV0.5), forced expiratory flow at 75% of FVC (FEF75), forced expiratory flow at 25%–75% of FVC (FEF25–75), and FEV0.5/FVC. Lung function was measured according to the raised volume rapid thoracoabdominal compression technique. In addition, a targeted analysis of six endogenous VOCs (acetone, isoprene, decane, undecane, tetradecane, and pentadecane) in the exhaled breath of the children was carried out by means of thermal desorption coupled gas chromatography‐single quadrupole mass spectrometry system. Results: A negatively significant relationship has been observed between levels of acetone, tetradecane, and pentadecane in exhaled breath and several of the lung function parameters. Levels of acetone (feature m/z = 58) were significantly nega tively associated with FVC and FVE0.5, levels of tetradecane (feature m/z = 71) with FEV0.5, and levels of pentadecane (feature m/z = 71) with FEV0.5 and FEF25–75. Conclusion: The findings of this study highlight a significant association between VOCs related to oxidative stress and lung function in healthy infants.
Open Access
Bacterial sirtuins overview: an open niche to explore
(Frontiers, 2021-10-26) Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Ortega Retuerta, Álvaro; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
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Engineering of microbial cell factories for production of plant-based natural products
(Elsevier, 2021) Gallego Jara, Julia; Lozano Terol, Gema; Sola Martínez, Rosa Alba; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Bioquímica y Biología Molecular B e Inmunología; Facultades de la UMU::Facultad de Medicina
Biotechnology has become a promising alternative to produce highly valuable products. Production using microorganisms competes with chemical synthesis and extraction from natural sources, leading to cheaper and more sustainable production. To produce natural plant products using microorganisms, the development of molecular biology techniques that allow us to genetically and metabolically modify host microorganisms is essential. Thus many biotechnological processes have been developed to obtain terpenes, alkaloids, or polyphenols with interesting applications in the pharmaceutical, food, or cosmetic industries. The increase in our knowledge regarding the metabolism of host organisms, together with the continuous development of genetic and metabolic engineering techniques, will allow, in the coming years, biotechnology to be positioned as the main way to obtain high-value plant natural products.
Open Access
Data preprocessing workflow for exhaled breath analysis by GC/MS using open sources
(2020) Pastor Hernández, José María; Lozano-Terol, Gema; García-Marcos Álvarez, Luis Vicente; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
The noninvasive diagnosis and monitoring of high prevalence diseases such as cardiovascular diseases, cancers and chronic respiratory diseases are currently priority objectives in the area of health. In this regard, the analysis of volatile organic compounds (VOCs) has been identified as a potential noninvasive tool for the diagnosis and surveillance of several diseases. Despite the advantages of this strategy, it is not yet a routine clinical tool. The lack of reproducible protocols for each step of the biomarker discovery phase is an obstacle of the current state. Specifically, this issue is present at the data preprocessing step. Thus, an open source workflow for preprocessing the data obtained by the analysis of exhaled breath samples using gas chromatography coupled with single quadrupole mass spectrometry (GC/MS) is presented in this paper. This workflow is based on the connection of two approaches to transform raw data into a useful matrix for statistical analysis. Moreover, this workflow includes matching compounds from breath samples with a spectral library. Three free packages (xcms, cliqueMS and eRah) written in the language R are used for this purpose. Furthermore, this paper presents a suitable protocol for exhaled breath sample collection from infants under 2 years of age for GC/MS.
Open Access
Comunicaciones Orales.- Structural analysis with DSC and Fluorescence in different mutants in lysine 101 of Catabolite Regulator Protein from Escherichia coli.
(2020-05-05) Écija Conesa, Ana; Arturo, Manjón Rubio; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología
Open Access
Regulation of the pyrimidine biosynthetic pathway by lysine acetylation of E. coli OPRTase
(2022-08-22) Lozano Terol, G.; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Martínez Vivancos, Adrián; Ortega Retuerta, Álvaro; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Open Access
Dynamic Lysine Acetylation Disrupts Isocitrate Lyase Function and Enables Metabolic Optimisation
(Wiley, 2026-03-30) Martínez Vivancos, Adrián; Gomariz-Turpin, Beatriz; Ortega Retuerta, Álvaro; Lozano Terol, Gema; Sola Martínez, Rosa Alba; Gallego Jara, Julia; Diego Puente, Teresa de; Bioquímica y Biología Molecular B e Inmunología
Proteomic studies have suggested that Escherichia coli isocitrate lyase (ICL) undergoes multiple acetylation events, partially inhibiting its activity. However, the molecular basis of this regulation and the contribution of individual lysine residues had not been defined. This study demonstrates that acetylation of ICL in E. coli is acetyl-phosphate–dependent and reversible by the CobB deacetylase, establishing a key post-translational regulatory mechanism within the glyoxylate shunt. Site-specific acetylation at K13 and K308 inhibits ICL activity by destabilising the tetrameric assembly and rendering the protein more prone to degradation, whereas lysine-to-arginine substitutions at these positions alleviate this inhibition, enhancing carbon flux distribution, metabolic flexibility and biomass yield without the burden of plasmid-based overexpression. Leveraging this regulatory insight, a KR mutant bearing lysine-to-arginine substitutions at residues 13 and 308, engineered directly into the chromosomal aceA gene, maintained wild-type growth rates while reducing acetate overflow and improving metabolic balance during glucose depletion and acetate assimilation, leading to a 61% increase in lycopene production. These findings highlight regulatory-based metabolic engineering as a powerful strategy to optimise bioproduction and pave the way for extending this approach to other central metabolic enzymes to develop robust microbial cell factories for the sustainable synthesis of biofuels, biochemicals and high-value compounds.
Open Access
Bacterial sirtuins overview: an open niche to explore
(Frontiers Media, 2021-10-26) Lozano Terol, Gema; Cánovas Díaz, Manuel; Diego Puente, Teresa de; Gallego Jara, Julia; Ortega Retuerta, Álvaro; Sola Martínez, Rosa Alba; Bioquímica y Biología Molecular B e Inmunología
Sirtuins are deacetylase enzymes widely distributed in all domains of life. Although for decades they have been related only to histones deacetylation in eukaryotic organisms, today they are considered global regulators in both prokaryotes and eukaryotes. Despite the important role of sirtuins in humans, the knowledge about bacterial sirtuins is still limited. Several proteomics studies have shown that bacterial sirtuins deacetylate a large number of lysines in vivo, although the effect that this deacetylation causes in most of them remains unknown. To date, only the regulation of a few bacterial sirtuin substrates has been characterized, being their metabolic roles widely distributed: carbon and nitrogen metabolism, DNA transcription, protein translation, or virulence. One of the most current topics on acetylation and deacetylation focuses on studying stoichiometry using quantitative LC-MS/MS. The results suggest that prokaryotic sirtuins deacetylate at low stoichiometry sites, although more studies are needed to know if it is a common characteristic of bacterial sirtuins and its biological significance. Unlike eukaryotic organisms, bacteria usually have one or few sirtuins, which have been reported to have closer common ancestors with the human Sirt5 than with any other class. In this work, in addition to carrying out an in-depth review of the role of bacterial sirtuins in their physiology, a phylogenetic study has been performed that reveals the evolutionary differences between sirtuins of different bacterial species and even between homologous sirtuins.
Open Access
Impact of environmental exposures on exhaled breath and lung function: NELA Birth Cohort
(ERS publications, 2025) Sola-Martínez, Rosa A.; Jiménez Guerrero, Pedro; Sánchez-Solís de Querol, Manuel; Lozano Terol, Gema; Gallego Jara, Julia; Martínez Vivancos, Adrián; Morales Bartolomé, Eva; García-Marcos Álvarez, Luis Vicente; Diego Puente, Teresa de; NELA Study Group; Cirugía, Pediatría y Obstetricia y Ginecología; Facultad de Medicina
Introduction: Exposure to environmental factors (i.e. air pollution and second-hand tobacco smoke) have been associated with impaired lung function. However, the impact of environmental factors on lung health is usually evaluated separately and not with an exposomic framework. In this regard, breath analysis could be a noninvasive tool for biomonitoring of global human environmental exposure. Methods: Data come from 337 mother-child pairs from the Nutrition in Early Childhood Asthma (NELA) birth cohort. Levels of BTEX (benzene, toluene, ethylbenzene and xylenes) in exhaled breath from mothers and children at 3 months after birth were estimated using gas hromatography-mass spectrometry. Short-term residential exposures (breath sampling day and 15 days before breath sampling) to nitrogen dioxide, particulate matter (PM2.5) and ozone were determined by chemical dispersion/transport modelling. Forced vital capacity, forced expiratory volume in 0.5 s (FEV0.5) and forced expiratory flow at 75% of FVC and at 25%-75% of FVC were measured in infants according to the raised-volume rapid thoracoabdominal compression technique. Results: The results showed significant associations between short-term exposure to external agents and levels of benzene and toluene in exhaled breath. It was observed that exhaled levels of benzene and toluene were influenced by smoking status and outdoor air pollution in mothers, and by air pollution in infants (3 months of age). No significant relationship was observed between exposure to maternal tobacco smoking and/or short-term air pollution and lung function in healthy infants. However, there was a significant relationship between FEV0.5 and exhaled toluene in children. Discussion: These findings indicated a significant relationship between environmental exposures and exhaled levels of benzene and toluene, suggesting that breath analysis could be a helpful exposure biomonitoring tool.