Browsing by Subject "oxidative stress"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Carbamazepine, cadmium chloride and polybrominated diphenyl ether-47, synergistically modulate the expression of antioxidants and cell cycle biomarkers, in the marine fish cell line SAF-1
    (Elsevier, 2019-11-21) Espinosa Ruiz, Cristóbal; Manuguerra, Simona; Curcuraci, Eleonora; Santulli, Andrea; Messina, Concetta M.; Biología Celular e Histología
    A wide range of contaminants, industrial by-products, plastics, and pharmaceutics belonging to various categories, have been found in sea water. Although these compounds are detected at concentrations that might be considered as sub-lethal, under certain conditions they could act synergistically producing unexpected effects in term of toxicity or perturbation of biochemical markers leading to standard pathway. In this study, the Sparus aurata fibroblast cell line SAF-1, was exposed to increasing concentrations of carbamazepine (CBZ), polybrominated diphenyl ether 47 (BDE-47) and cadmium chloride (CdCl2) until 72 h, to evaluate the cytotoxicity and the expression of genes related to antioxidant defense, cell cycle and energetic balance. In general, both vitality and gene expression were affected by the exposure to the different toxicants, in terms of antioxidant defense and cell cycle control, showing the most significant effects in cells exposed to the mixture of the three compounds, respect to the single compounds separately. The synergic effect of the compounds on the analyzed biomarkers, underlie the potential negative impact of the contaminants on health of marine organisms.
  • Thumbnail Image
    Publication
    Open Access
    Effects of Commercial Antioxidants in Feed on Growth Performance and Oxidative Stress Status ofWeaned Piglets
    (MDPI AG, 2021-01-21) Orengo Femenia, Juan; Hernández Ruipérez, Fuensanta; Martínez Miró, Silvia; Sánchez Parra, Cristian Jesús; Peres Rubio, Camila; Madrid Sánchez, Josefa; Producción Animal
    This work aimed to evaluate the effect of adding two different commercial antioxidants (AOX) products to pre-starter and starter diets using low vitamin E (Vit E as DL- -tocopheryl acetate) levels on the growth performance and oxidative stress of piglets for the first six weeks post-weaning (PW). They were sorted by initial body weight (BW: 6.175 0.931 kg) and randomly allotted to four dietary treatments (with six replicates per treatment): a positive control (PC) and a negative control (NC) diet, with normal and low dose of vitamin E (80 and 15 mg kg􀀀1, respectively), both without AOX; the other two experimental diets with a low dose of vitamin E (LVE) plus LOXIDAN VD100 (LVE + AOX1) or LOXIDAN E Ros (LVE + AOX2). Growth data were recorded, and blood samples were taken, at the beginning (day 0) and at the end of each feeding period: pre-starter and starter (at days 14 and 42, respectively). No differences among dietary treatments were found with respect to growth performance in the pre-starter period (p 0.05). However, at the end of the starter period, a lower BW was found in piglets fed the NC diet compared to the other dietary treatments. Differences in daily gain and feed conversion ratio were also found either for the starter period or when the whole period was considered (p < 0.05), whereby piglets fed PC or LVE diets supplemented with AOX showed better growth performance compared to piglets fed the NC diet. Regarding Vit E ( -tocopherol) serum levels, there were no differences among treatments at day 0; but the serum values of this vitamin decreased in LVE diets at 14 and 42 days, but not in the PC. On day 42, the highest levels of -tocopherol in liver were also found in piglets fed PC (p < 0.05). Nevertheless, in general, from a metabolic point of view and after checking the serum biochemical profile of piglets, there were no differences in other oxidative stress markers (p 0.05). The results showed that the AOX products used were able to compensate for the lower Vit E supply with respect to growth performance in the starter phase. The use of AOXs or usual levels of Vit E in feed constitutes a key factor in achieving optimal growth performance of piglets in the PW period.
  • Thumbnail Image
    Publication
    Open Access
    Sub-lethal doses of polybrominated diphenyl ethers affect some biomarkers involved in energy balance and cell cycle, via oxidative stress in the marine fish cell line SAF-1
    (Elsevier, 2019-02-19) Espinosa Ruiz, Cristóbal; Manuguerra, Simona; Santulli, Andrea; Messina, Concetta M.; Cuesta Peñafiel, Alberto; Esteban Abad, María de los Ángeles; Biología Celular e Histología
    Polybrominated diphenyl ethers (PBDEs) are a class of persistent contaminants which are found all over the world in the marine environment. Sparus aurata fibroblast cell line (SAF-1) was exposed to increasing concentrations of PBDEs 47 and 99, until 72 hours to evaluate the cytotoxicity, reactive oxygen species (ROS) production and the expression of some selected molecular markers related to cell cycle, cell signaling, energetic balance and oxidative stress (p53, erk-1, hif-1α and nrf-2), by real-time PCR. Furthermore, SAF-1 cells were exposed for 7 and 15 days to sub-lethal concentrations, in order to evaluate the response of some biomarkers by immunoblotting (p53,ERK-1, AMPK, HIF-1α and NRF-2). After 48 and 72 hours, the cells showed a significant decrease of cell vitality as well as an increase of intracellular ROS production. Gene expression analysis showed that sub-lethal concentrations of BDE-99 and 47, after 72 hours, up-regulated cell cycle and oxidative stress biomarkers, although exposure to 100μmol L-1 down-regulated the selected markers related to cell cycle, cell signaling, energetic balance. After 7 and 15 days of sub-lethal doses exposure, all the analyzed markers resulted affected by the contaminants. Our results suggest that PBDEs influence the cells homeostasis first of all via oxidative stress, reducing the cell response and defense capacity and affecting its energetic levels. This situation of stress and energy imbalance could represents a condition that, modifying some of the analyzed biochemical pathways, would predispose to cellular transformation.