Browsing by Subject "Zn"

Now showing 1 - 3 of 3
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    Analytical characterization of trace elements (zinc, copper, cadmium, lead and selenium) in saliva of pigs under common pathological conditions in the field: a pilot study
    (BMC [Commercial Publisher], 2020) Sánchez, Jorge; Montilla, Miguel; Gutiérrez-Panizo, Cándido; Sotillo, Juan; Fuentes, Pablo; Montes, Ana; Gutiérrez, Ana María; Medicina y Cirugía Animal
    Background: This study is focused on the measurement of trace elements (zinc, copper, cadmium, lead and selenium) in the saliva of pigs in order to study their levels on different porcine pathological conditions in the field. The experiment involved 15 pigs without clinical signs of disease and 42 diseased pigs (suffering from lameness, rectal prolapse, fatigue or growth rate retardation). Individual saliva samples were collected, allowing the pigs to chew a sponge each for trace element quantifications through atomic absorption spectrometry (AAS). Since this is the first report on the measurements of trace elements in porcine saliva, a routine analytical validation study was performed for the quantification of all the studied elements. Moreover, the acute phase proteins C-reactive protein (CRP) and haptoblobin (Hp), the total antioxidant capacity (TAC) and adenosine deaminase (ADA) were quantified in the saliva samples for the animal’s health status assessment. Results: Modifications in the levels of acute phase proteins or ADA were only recorded in animals with lameness and rectal prolapse and those with fatigue respectively. Moreover, TAC level changes were observed in pigs with growth-rate retardation. However, alterations in the levels of two or more trace elements were reported for all the different groups of diseased pigs with evident variations within pathologies. Conclusions: The salivary quantification of trace elements could be considered as a complementary tool to acute phase proteins, TAC and ADA determinations for disease detection and differentiation in the pig and should be explored in greater depth.
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    Fast determination of the Ca, Mg, K, Na and Zn contents in milk and nondairy imitation milk using ICP-AES without mineralization stage
    (Elsevier Academic Press, 1999-05) Murcia Tomás, María Antonia; Vera, Ana; Martínez-Tomé, Magdalena; Muñoz, Antonio; Hernández-Córdoba, Manuel; Ortiz-Gonzalez, Roque; Tecnología de Alimentos, Nutrición y Bromatología
    The mineral elements Ca, Mg, K, Na and Zn were analysed directly in diluted milk and nondairy imitation milk using inductively coupled plasma atomic emission spectrometry without previous treatment except dilution. The results obtained agreed with those obtained using the AOAC 984.27/90 mineralization stage. The data prove that for the routine control analysis of these elements the conventional mineralization stage is not needed, the dilution of the samples being suzcient to overcome matrix ewects.
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    Influence of the circadian cycle, sex and production stage on the reference values of parameters related to stress and pathology in porcine saliva
    (Springer Nature, 2023) Saco, Y.; Peña, R.; Matas-Quintanilla, M.; Ibáñez-López, F. J.; Piñeiro, M.; Sotillo, J.; Bassols, A.; Gutiérrez, A. M.; Medicina y Cirugía Animal
    Background The concentration of biomarkers in saliva could be influenced by several factors not related to the specific condition under analyses, which should be considered for proper clinical interpretation. In the present study, the circadian rhythm of C-reactive protein (CRP), haptoglobin (Hp), Pig-MAP, S100A12, Cu, Zn, Adenosine deaminase (ADA), total protein (TP), total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), cortisol and α-amylase in saliva of 20 female and 20 male pigs was investigated. Moreover, the influence of sex and production phase (post-weaning, fattening and finishing) on the concentrations of biomarkers in a total of 414 healthy pigs was studied and the reference intervals for all salivary biomarkers were calculated accordingly. Results All parameters except Pig-MAP, OSI and α-amylase varied significantly along the daytime, and most of them peak around early afternoon (13–15 h). The cosinor analysis described the temporal dynamics of circadian rhythms for all parameters. The range values showed differences between male and female pigs in 8 out of the 13 biomarkers, with higher concentrations in females in comparison to male pigs. The influence of the production phase on the salivary concentrations was observed for all the biomarkers. The highest concentrations were observed for Pig-MAP, S100A12 and α-amylase in post-weaning animals, for TP in growing pigs and for OSI in finishing animals. Most of the sex-influenced biomarkers showed the highest concentrations at growing stages with some exceptions such as ADA or Hp that showed the peak at finishing and post-weaning stages respectively. Conclusions It is necessary to establish the optimal daytime for routine saliva sampling to avoid circadian variations and for that end, the time interval between 10:00 a.m. to 12:00 a.m. is highly recommended. The factors sex and production phase influence the concentration of biomarkers and should be considered for proper biomarker interpretation. The reference intervals presented here for each salivary biomarker will help to correctly interpret the results of these analytes and contribute to the use of saliva as a non-invasive sample for the diagnosis and monitoring of the health status of swine farms.