Browsing by Subject "Transcriptome"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs
    (2019-12-18) Martínez, Cristina A.; Cambra, Josep M.; Parrilla, Inmaculada; Roca, Jordi; Ferreira-Dias, Graça; Pallarés, Francisco J.; Lucas, Xiomara; Vázquez, Juan M.; Martínez, Emilio A.; Gil, María A.; Rodríguez-Martínez, Heriberto; Cuello, Cristina; Álvarez-Rodríguez, Manuel; Medicina y Cirugía Animal
    Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, and potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected embryo development and the transcriptional pattern of the pig endometria containing embryos. Materials and Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine infusions of either heterologous pooled SP or Beltsville Thawing Solution (BTS; control) 30 min before AI of semen extended to 10% of homologous SP. Embryos (all sows) and endometrium samples (3 sows/group) were removed during laparotomy 6 days after the infusion of SP or BTS to morphologically evaluate the embryos to determine their developmental stage and to analyze the endometrial transcriptome using microarrays (PORGENE 1.0 ST GeneChip array, Affymetrix) followed by qPCR for further validation. Results: Embryo viability was equal between the groups (~93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others. Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.
  • Thumbnail Image
    Publication
    Open Access
    Seminal plasma modulates miRNA expression by sow genital tract lining explants
    (MDPI, 2020-06-19) Barranco, Isabel; Padilla, Lorena; Martínez, Cristina A.; Álvarez-Rodríguez, Manuel; Parrilla, Inmaculada; Lucas, Xiomara; Ferreira-Dias, Graça; Yeste, Marc; Rodríguez-Martínez, Heriberto; Roca, Jordi; Medicina y Cirugía Animal
    The seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in the process. Considering that the boar ejaculate is built by fractions differing in SP-composition, this study evaluated whether exposure of mucosal explants of the sow internal genital tract (uterus, utero-tubal junction and isthmus) to different SP-fractions changed the profile of explant-secreted miRNAs. Mucosal explants retrieved from oestrus sows (n = 3) were in vitro exposed to: Medium 199 (M199, Control) or M199 supplemented (1:40 v/v) with SP from the sperm-rich fraction (SRF), the post-SRF or the entire recomposed ejaculate, for 16 h. After, the explants were cultured in M199 for 24 h to finally collect the media for miRNA analyses using GeneChip miRNA 4.0 Array (Affymetrix). Fifteen differentially expressed (False Discovery Rate (FDR) < 0.05 and Fold-change ≥ 2) miRNAs (11 down- versus 4 up-regulated) were identified (the most in the media of uterine explants incubated with SP from post-SRF). Bioinformatics analysis identified that predicted target genes of dysregulated miRNAs, mainly miR-34b, miR-205, miR-4776-3p and miR-574-5p, were involved in functions and pathways related to immune response. In conclusion, SP is able to elicit changes in the miRNAs profile secreted by female genital tract, ultimately depending SP-composition.
  • Thumbnail Image
    Publication
    Open Access
    Upregulation and epigenetic modification of the creatine transporter SLC6A8 in non-small cell lung cancer
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Kuempers, Christiane; Schnepf, Karoline; Marwitz, Sebastian; Watermann, Christian; Scheel, Andreas H.; Fischer, Rieke N.; Ammerpohl, Ole; Perner, Sven; Drömann, Daniel; Goldmann, Torsten
    Introduction. Lung cancer is a major cause of cancer-related death worldwide and effective therapies, besides surgery, are available only for a small proportion of patients. Since cellular respiration is known to be broadly altered in malignant tumors, the cellular processes of respiration can be a potential therapeutic target. One important element of cellular respiration is creatine and its transport by the creatine transporter SLC6A8. Here we describe the expression of SLC6A8 at the RNA and protein level, epigenetic modifications as well as survival analysis in NSCLC tissues and matched controls. Materials and Methods. We analyzed epigenetic modifications of the SLC68A gene in 32 patients, of which 18 were additionally analyzed by transcriptome analysis. The expression of SLC6A8 at the protein level was assessed by immunohistochemistry using an independent cohort and correlated with clinico-pathological data including survival. Kaplan-Meier analysis was performed to analyze the possible effects of the transcriptional levels of SLC6A8 in another separate cohort (n=1925). Results. SLC6A8 loci are epigenetically modified in NSCLC compared with tumor-free controls. SLC6A8 is upregulated in NSCLC at the RNA and protein level. High mRNA expression of SLC6A8 was associated with an overall poor prognosis in lung adenocarcinoma patients and displayed the strongest adverse prognostic effect in male smokers with adenocarcinomas. Results of transcriptome analysis were partially confirmed at the protein level. Conclusions. Our results suggest an important role of creatine and its transport via SLC6A8 in NSCLC.