Browsing by Subject "Testosterona"

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    Differential actions of eplerenone and spironolactone on the protective effect of testosterone against cardiomyocyte apoptosis in vitro
    (2010-07) Sanchez-Mas, Jesus; Turpin, Maria C; Lax Pérez, Antonio Manuel; Ruiperez, Juan A; Valdes Chavarri, Mariano; Pascual Figal, Domingo A.; Medicina
    Introduction and objectives: Testosterone deficiency is associated with a poor prognosis in patients with heart failure. It is not clear whether testosterone reduces cardiomyocyte apoptosis or whether the effect of spironolactone, an aldosterone receptor blocker with progestogenic and anti-androgen activity, differs from that of the selective aldosterone blocker eplerenone. Methods: Apoptosis induced by hyperosmotic stress in the embryonic rat heart cell line H9c2 was monitored by measuring cell viability, DNA fragmentation and caspase-3, -8 and -9 activation. The effect of testosterone was investigated in the presence or absence of spironolactone and eplerenone. Results: Exposure to sorbitol (0.6 M, 3 h) decreased cell viability and increased DNA fragmentation and caspase-3, -8 and -9 activation. These effects were all significantly reduced by testosterone, 100 nM (P< .01). Pretreatment with spironolactone, 10 .M, blocked the effects of testosterone, decreased cell viability (P< .01) and increased caspase activation (P< .01). In contrast, eplerenone, 10 .M, increased cell viability (P< .001) without altering the effect on caspase activation. These actions were not modified by the androgen receptor blocker flutamide. They were mediated by SAPK/JNK and ERK1/2 signaling pathways (P< .01). Conclusions: Testosterone appears to have a protective effect against cardiomyocyte apoptosis which is antagonized by spironolactone but not by eplerenone. These effects await confirmation in in vivo models, but their presence could have clinical and therapeutic implications.
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    La ratio interdigital D2:D4 y su relación con otros indicadores de androgenización en progenitores de personas con trastornos del espectro autista
    (Murcia: Universidad de Murcia, Editum, 2013-01) Romero-Martínez, Ángel; de Andrés-García, Sara; Sariñana-González, Patricia; Sanchis-Calatayud, M.V.; Roa, Juan M.; González-Bono, Esperanza; Moya-Albiol, L.
    La ratio D2:D4 es el cociente entre la longitud de los dedos índice y anular e indicador indirecto de androgenización. A mayor testosterona prenatal,incrementa la probabilidad de desarrollar una menor ratio D2:D4. Se ha sugerido que personas con trastornos del espectro autista (TEA) y sus progenitores podrían tener cerebros altamente androgenizados, por lo que se ha utilizado como marcador de tal idiosincrasia. El objetivo principal es analizar si los progenitores de personas con TEA difieren de la población general en diversos parámetros de androgenización, principalmente la ratio D2:D4. La muestra estuvo compuesta por 43 progenitores de personas con TEA y 42 controles, de los que se midieron la ratio D2:D4, cuestionarios rasgo y niveles de testosterona y cortisol en saliva. Aunque no se encontraron diferencias por grupo en la ratio D2:D4, la ratio de la mano izquierda de progenitores de personas con TEA mostró mayor capacidad predictiva para explicar los cocientes de empatía y autismo, conductas cooperativas y niveles de cortisol. Además, en los progenitores varones predijo la gravedad de los hijos. Los resultados obtenidos indican que la ratio D2:D4 podría ser empleada, junto a otros parámetros, como indicador de la probabilidad de desarrollar rasgos autistas en los descendientes.